Tg(TG-BRAF*V600E)2Jaf
Transgene Detail

Summary

• Symbol: Tg(TG-BRAF*V600E)2Jaf
• Name: transgene insertion 2, James A Fagin
• MGI ID: MGI:5524234
• Synonyms: Tg-BRAF2
• Transgene: Tg(TG-BRAF*V600E)2Jaf Location: unknown
• Alliance: Tg(TG-BRAF*V600E)2Jaf page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TG-BRAF*V600E)2Jaf expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  BRAF (673)

  Braf	(MGI:88190)

  Transgene codes for a protein with a valine to glutamic acid mutation.

• Mutation details: The human BRAF cDNA with a thymine-to-adenine transversion at position 1799A leading to a valine-to-glutamate substitution at residue 600 (V600E) and an NH2-terminal myc tag are under the control of the thyroid-specific bovine thyroglobulin promoter. Quantitative RT-PCR and Western blot analysis confirmed mRNA and protein expression in thyroid tissue. This line is the higher expressing line. Males show higher levels of expression than females. (J:98550)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:98550 Knauf JA, et al., Targeted expression of BRAFV600E in thyroid cells of transgenic mice results in papillary thyroid cancers that undergo dedifferentiation. Cancer Res. 2005 May 15;65(10):4238-45
• All: 4 reference(s)