Tg(Amh-SMAD2*)2Tkw
Transgene Detail

Summary

• Symbol: Tg(Amh-SMAD2*)2Tkw
• Name: transgene insertion 2, Teresa K Woodruff
• MGI ID: MGI:5528705
• Synonyms: MIS-Smad2-dn, Smad2-dn
• Transgene: Tg(Amh-SMAD2*)2Tkw Location: unknown
• Alliance: Tg(Amh-SMAD2*)2Tkw page

Transgene origin

• Strain of Origin: CD-1

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Amh-SMAD2*)2Tkw expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SMAD2 (4087)

  Smad2

  (MGI:108051)

  Expresses a a C-terminal truncation of the human SMAD2 protein

• Mutation details: This transgene consists of a mouse minimal Mullerian-inhibiting substance promoter (-180 bp), an epitope Flag tag, a C-terminal truncation of the human Smad2 gene resulting in a dominant negative effect, and a human GH polyadenylation sequence. The Amh promoter drives transgene expression in testicular Sertoli cells and ovarian granulosa cells. RT-PCR and immunolocalization confirmed expression of RNA and protein in ovaries. Three lines were generated: 1, 2, and 3, however line 1 was lost (J:129226)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:129226 Bristol-Gould SK, et al., The development of a mouse model of ovarian endosalpingiosis. Endocrinology. 2005 Dec;146(12):5228-36
• All: 1 reference(s)