Tg(JAK2*V617F)FF1Rsko
Transgene Detail

Summary

• Symbol: Tg(JAK2*V617F)FF1Rsko
• Name: transgene insertion FF1, Radek C Skoda
• MGI ID: MGI:5553468
• Synonyms: JAK2-V617F
• Transgene: Tg(JAK2*V617F)FF1Rsko Location: unknown Genetic Position: Chr8, Syntenic
• Alliance: Tg(JAK2*V617F)FF1Rsko page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(JAK2*V617F)FF1Rsko expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  JAK2 (3717)

  Jak2	(MGI:96629)

• Mutation details: The human BAC, CTD2025A15, containing JAK2 exons 1-12 was combined with a partial cDNA encoding JAK2 exons 13-25 containing the V617F mutation and a polyadenylation signal. To create an inducible Flip-Flop transgene, this construct was modified by inserting a lox66 site into intron 12 and a lox71 site after the polyadenylation signal and the segment between the loxP sites was inverted. Cre-recombination of antiparallel loxP sites flips the orientation of the cDNA and places the intron 12 splice acceptor into the correct position to allow proper splicing of the transgenic pre-mRNA. Recombination generates one wild-type loxP and one double mutant lox66/77 site, which is no longer a substrate for the cre recombinase. This line carries 9 copies of the transgene that integrated in a single chromosomal locus on chromosome 8, band A1. (J:134364)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:134364 Tiedt R, et al., Ratio of mutant JAK2-V617F to wild-type Jak2 determines the MPD phenotypes in transgenic mice. Blood. 2008 Apr 15;111(8):3931-40
• All: 16 reference(s)