Tg(UBC-GFP/HTT*84Q)22Shya
Transgene Detail

Summary

• Symbol: Tg(UBC-GFP/HTT*84Q)22Shya
• Name: transgene insertion 22, Shang-Hsun Yang
• MGI ID: MGI:5565690
• Synonyms: Ubi-G-HTT84Q
• Transgene: Tg(UBC-GFP/HTT*84Q)22Shya Location: unknown
• Alliance: Tg(UBC-GFP/HTT*84Q)22Shya page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(UBC-GFP/HTT*84Q)22Shya expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HTT (3064)

  Htt	(MGI:96067)

• Mutation details: This transgene consists of exon 1 of the human HTT with the expansion of 84 CAG trinucleotide repeats, which encodes 153 amino acids, in the 3' end of the GFP gene, where the stop codon was removed and mutant HTT was ligated in frame, under the control of the human polyubiquitin-C promoter. Western blotting confirmed protein expression in the heart, liver, lung, spleen, kidney, muscle, and brain, with highest levels in the brain. GFP expression was confirmed in the brain. (J:209055)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:209055 Cheng PH, et al., Significantly differential diffusion of neuropathological aggregates in the brain of transgenic mice carrying N-terminal mutant huntingtin fused with green fluorescent protein. Brain Struct Funct. 2013 Jan;218(1):283-94
• All: 2 reference(s)