Tg(ASL1/Cryaa-Ralbp1)24Yca
Transgene Detail

Summary

• Symbol: Tg(ASL1/Cryaa-Ralbp1)24Yca
• Name: transgene insertion 24, Yogesh C Awasthi
• MGI ID: MGI:5572816
• Synonyms: lensRLIP76 Tg
• Transgene: Tg(ASL1/Cryaa-Ralbp1)24Yca Location: unknown
• Alliance: Tg(ASL1/Cryaa-Ralbp1)24Yca page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(ASL1/Cryaa-Ralbp1)24Yca expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ralbp1 (MGI:108466)

• Mutation details: This transgene consists of the delta en alphaA promoter, which consists of a heterogenic enhancer/promoter comprising the third intron of the chick delta1-crystallin gene and the mouse alpha crystalline promoter, a rabbit beta-globin intron, mouse Ralbp1 cDNA (2884 bp) and bovine growth hormone polyadenylation signal. This enhancer/promoter becomes active at the lens pit stage and remains active in all lens cells including both lens epithelial and fiber cells. Western blot and immunofluorescence confirmed expression in epithelial and fiber cells. Five lines were established: 3, 4, 24, 41, and 47. Line 24 and 47 have the highest numbers of transgene copies. (J:210388)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:210388 Sahu M, et al., Lens specific RLIP76 transgenic mice show a phenotype similar to microphthalmia. Exp Eye Res. 2014 Jan;118:125-34
• All: 1 reference(s)