Tg(Myh6-KCNQ1_i2)H05Desc
Transgene Detail

Summary

• Symbol: Tg(Myh6-KCNQ1_i2)H05Desc
• Name: transgene insertion H05, Denis Escande
• MGI ID: MGI:5575891
• Synonyms: H05
• Transgene: Tg(Myh6-KCNQ1_i2)H05Desc Location: unknown
• Alliance: Tg(Myh6-KCNQ1_i2)H05Desc page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Dominant negative, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-KCNQ1_i2)H05Desc expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  KCNQ1 (3784)

  Kcnq1

  (MGI:108083)

• Mutation details: The human KCNQ1 isoform 2 is under the control of the mouse alpha-myosin heavy chain promoter (including introns, and three noncoding exons). The stop codon was removed and an 11-amino acid T7 epitope tag was added in frame at the carboxyl terminus of KCNQ1. Isoform 2 does not form functional channels but exerts dominant-negative effects on isoform 1 channels. Western blot analysis confirmed expression. This is the low expressing line. (J:132483)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:132483 Demolombe S, et al., Transgenic mice overexpressing human KvLQT1 dominant-negative isoform. Part I: Phenotypic characterisation. Cardiovasc Res. 2001 May;50(2):314-27
• All: 2 reference(s)