Tg(Prnp*D177N*M128V)G1Rchi
Transgene Detail

Summary

• Symbol: Tg(Prnp*D177N*M128V)G1Rchi
• Name: transgene insertion G1, Roberto Chiesa
• MGI ID: MGI:5614760
• Synonyms: Tg(CJD)
• Transgene: Tg(Prnp*D177N*M128V)G1Rchi Location: unknown
• Alliance: Tg(Prnp*D177N*M128V)G1Rchi page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA/J)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Nucleotide substitutions
• Tg(Prnp*D177N*M128V)G1Rchi expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Prnp (MGI:97769)	Transgene codes for a protein with aspartic acid to asparagine and methionine to valine mutations.

• Mutation details: The transgene construct contained a a 12 kb genomic DNA fragment of the mouse Prnp gene, including the promoter and first intron, joined to a cDNA encoding the a variant of mouse prion protein in which aspartic acid is replaced by asparagine at anino acid position 177 (D177N) and methionine by valine at position 128 (M128V), equivalent to the hereditary Creutzfeldt-Jakob disease- (CJD-) associated human D178N/V129 prion protein mutation. Hemizygous mice of transgenic line G1 carry 6 copies of the transgene; western blot analysis detects the mutant prion protein in brains of these mice at 0.15 times the endogenous PRNP level in wild-type brains. The gross pattern of mutant PRNP expression in brains of transgenic mice is similar to that of endogenous PRNP in non-transgenic mice. (J:142098)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:142098 Dossena S, et al., Mutant prion protein expression causes motor and memory deficits and abnormal sleep patterns in a transgenic mouse model. Neuron. 2008 Nov 26;60(4):598-609
• All: 1 reference(s)