Tg(SOD1*G85R/EYFP)641Alho
Transgene Detail

Summary

• Symbol: Tg(SOD1*G85R/EYFP)641Alho
• Name: transgene insertion 641, Arthur L Horwich
• MGI ID: MGI:5621057
• Synonyms: G85R SOD-YFP
• Transgene: Tg(SOD1*G85R/EYFP)641Alho Location: unknown
• Alliance: Tg(SOD1*G85R/EYFP)641Alho page

Gliosis in Tg(SOD1*G85R/EYFP)641Alho/Tg(SOD1*G85R/EYFP)641Alho mice

Show the 2 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: (C57BL/6J x SJL/J)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(SOD1*G85R/EYFP)641Alho expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SOD1 (6647)

  Sod1	(MGI:98351)

• Mutation details: A 12 kb genomic fragment of human SOD1 carrying the glycine to arginine substitution at amino acid 85 (G85R) fused to EYFP coding sequence through its C-terminal coding sequence in exon 5 was used as the transgene. The G85R mutation results in a misfolded protein associated with amyotrophic lateral sclerosis in humans and the mutant fusion protein behaves as a misfolded monomer. Southern blot analysis indicates that line 641 carries a single (multicopy) transgene insertion. In situ RNA hybridization indicates that the transgene is predominantly in motor neurons in the spinal cord. (J:144475)

Schematic of the Tg(SOD1*G85R/EYFP)641Alho transgene

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:144475 Wang J, et al., Progressive aggregation despite chaperone associations of a mutant SOD1-YFP in transgenic mice that develop ALS. Proc Natl Acad Sci U S A. 2009 Feb 3;106(5):1392-7
• All: 7 reference(s)