Tg(Ckm-Prkaa2*D157A)1Ljg
Transgene Detail
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Symbol: |
Tg(Ckm-Prkaa2*D157A)1Ljg |
Name: |
transgene insertion 1, Laurie J Goodyear |
MGI ID: |
MGI:5636710 |
Synonyms: |
alpha2i TG, AMPKalpha2i TG |
Transgene: |
Tg(Ckm-Prkaa2*D157A)1Ljg Location: unknown
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Alliance: |
Tg(Ckm-Prkaa2*D157A)1Ljg page
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Transgene Type: |
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Transgenic (Inserted expressed sequence) |
Mutation: |
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Insertion
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Tg(Ckm-Prkaa2*D157A)1Ljg expresses
1 gene
Transgene expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
rat |
Prkaa2 (78975) |
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Transgene codes for a protein with an aspartic acid to alanine mutation. |
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Mutation details: The transgenic construct contains a rat cDNA containing the Prkaa2 gene modified by site-directed mutagenesis to contain the D157A mutation under the control of the muscle creatine kinase promoter. The mutation changes the aspartic acid at residue 157 to alanine thus inactivating the catalytic alpha 2 subunit. The inactive alpha 2 subunit is tagged with a hemaglutinin epitope at the amino terminus. Expression of endogenous Prkaa2 is detectable only after very long exposure times and transgene expression occurs in skeletal muscle.
(J:104111)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:104111 Fujii N, et al., AMP-activated protein kinase alpha2 activity is not essential for contraction- and hyperosmolarity-induced glucose transport in skeletal muscle. J Biol Chem. 2005 Nov 25;280(47):39033-41 |
All: |
12 reference(s) |
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