Tg(CAG-lacZ,-FUS*R521G,-EGFP)682Gyu
Transgene Detail

Summary

• Symbol: Tg(CAG-lacZ,-FUS*R521G,-EGFP)682Gyu
• Name: transgene insertion 682, Gang Yu
• MGI ID: MGI:5644691
• Transgene: Tg(CAG-lacZ,-FUS*R521G,-EGFP)682Gyu Location: unknown
• Alliance: Tg(CAG-lacZ,-FUS*R521G,-EGFP)682Gyu page

Tg(CAG-lacZ,-FUS*R521G,-EGFP)682Gyu/0 Meox2^tm1(cre)Sor/Meox2⁺ mice display hindlimb curl

Show the 5 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(CAG-lacZ,-FUS*R521G,-EGFP)682Gyu expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  FUS (2521)

  Fus	(MGI:1353633)

• Mutation details: This transgene consists of the cytomegalovirus immediate early enhancer-chicken beta-actin hybrid (CAG) promoter, a floxed lacZ gene, human FUS cDNA with the R521C mutation, IRES, and EGFP coding sequences. Cre-excision of the loxP flanked lacZ DNA allows for translation of FUS and EGFP. Two lines were generated: 673, and 682. Line 682 was chosen as a representative line. (J:216672)

Generation of the Tg(CAG-lacZ,-FUS,-EGFP)629Gyu and Tg(CAG-lacZ,-FUS*R521G,-EGFP)682Gyu transgenes

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:216672 Sephton CF, et al., Activity-dependent FUS dysregulation disrupts synaptic homeostasis. Proc Natl Acad Sci U S A. 2014 Nov 4;111(44):E4769-78
• All: 3 reference(s)