Tg(SFTPC*)#Twv
Transgene Detail

Summary

• Symbol: Tg(SFTPC*)#Twv
• Name: transgene insertion, Timothy E Weaver
• MGI ID: MGI:5647890
• Synonyms: SP-C^deltaexon4
• Transgene: Tg(SFTPC*)#Twv Location: unknown
• Alliance: Tg(SFTPC*)#Twv page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(SFTPC*)#Twv expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SFTPC (6440)

  Sftpc

  (MGI:109517)

  Exon 4 is deleted from the expressed cDNA.

• Mutation details: A human SP-C delta exon4 cDNA was generated by deleting nucleotides 325435 (adenosine of start ATG is base pair 1). A transgene construct was generated by subcloning the human SP-C delta exon4 cDNA into a vector containing the 13-kb mouse SP-C promoter, a rabbit globin intronic/exonic sequences, and a bovine growth hormone polyadenylation signal. Two founder mice were generated but did not survive. (J:87084)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Two founder mice were generated but did not survive. No lines were established. The phenotypes of the founder mice are reported. Severity of the phenotype was correlated with the expression level of the mutated protein.

References

• Original: J:87084 Bridges JP, et al., Expression of a human surfactant protein C mutation associated with interstitial lung disease disrupts lung development in transgenic mice. J Biol Chem. 2003 Dec 26;278(52):52739-46
• All: 1 reference(s)