Tg(tetO-PTPN11*E76K)#Jiwu
Transgene Detail

Summary

• Symbol: Tg(tetO-PTPN11*E76K)#Jiwu
• Name: transgene insertion, Jie Wu
• MGI ID: MGI:5688299
• Synonyms: tetO-SHP2^E76K
• Transgene: Tg(tetO-PTPN11*E76K)#Jiwu Location: unknown
• Alliance: Tg(tetO-PTPN11*E76K)#Jiwu page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Constitutively active, Humanized sequence, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-PTPN11*E76K)#Jiwu expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PTPN11 (5781)

  Ptpn11

  (MGI:99511)

• Mutation details: The chicken beta-globin insulator sequence and tetracycline operator are used to drive inducible expression of a constitutively active human PTPN11 containing the E76K mutation with a C-terminal Flag tag. A pair of oppositely oriented heterotypic L3 and L2 loxP sites flank the transgenic cassette. Three lines that show no leaky expression of the transgene were created: 398, 425, and 417. However, the pound symbol (#) is used when the line is not specified and/or lines are pooled. (J:212505)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:212505 Schneeberger VE, et al., SHP2E76K mutant promotes lung tumorigenesis in transgenic mice. Carcinogenesis. 2014 Aug;35(8):1717-25
• All: 2 reference(s)