Tg(LONP1-mt-ND4*,-mCherry*)#Jguy
Transgene Detail

Summary

• Symbol: Tg(LONP1-mt-ND4*,-mCherry*)#Jguy
• Name: transgene, John Guy
• MGI ID: MGI:5689425
• Synonyms: mutND4, sc-HSP-mutantND4FLAG-mt-mCherry
• Transgene: Tg(LONP1-mt-ND4*,-mCherry*)#Jguy Location: unknown
• Alliance: Tg(LONP1-mt-ND4*,-mCherry*)#Jguy page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(LONP1-mt-ND4*,-mCherry*)#Jguy expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ND4 (4538)

  mt-Nd4

  (MGI:102498)

• Mutation details: The mitochondria-targeted self-complementary adeno-associated virus (AAV) transgene contained the human mitochondrial heavy-strand promoter driving mitochondrial expression of the FLAG tagged human mt-ND4 with the substitution of A for G taht results in an arginine-to-histidine substitution and mitochondrial mCherry with the substitution A for C. This mutation is responsible for Leber hereditary optic neuropathy (LHON). There are two stop codons between the genes. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:224897)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

The transgene is maintained without integration into the host genome.

References

• Original: J:224897 Yu H, et al., Consequences of zygote injection and germline transfer of mutant human mitochondrial DNA in mice. Proc Natl Acad Sci U S A. 2015 Oct 20;112(42):E5689-98
• All: 1 reference(s)