Pas15 QTL Detail

Summary

• Symbol
  Pas15
• Name
  pulmonary adenoma susceptibility 15

• Feature Type
  QTL
• IDs
  MGI:5699075
• Candidate Genes
  6 Genes

Location &
Maps

• Sequence Map
  Chr6:145180868-145181064 bp

  From MGI annotation of GRCm39
  

• View this region in JBrowse
  

• Genome Browsers
  Ensembl | UCSC | NCBI

• Genetic Map
  Chromosome 6, cM position of peak correlated region/marker: Syntenic
• Mapping Data
  5 experiments

Mutations,
Alleles, and
Phenotypes

• Phenotype Summary
  3 phenotype references
  

• All Mutations and Alleles
  2
• QTL
  2
• Find Mice (IMSR)
  0 strains or lines available

References

• Summaries
  All 5

  Phenotypes 3
• Earliest
  J:22173 Devereux TR, et al., Assignment of a locus for mouse lung tumor susceptibility to proximal chromosome 19. Mamm Genome. 1994 Dec;5(12):749-55
• Latest
  J:108212 Galbiati F, et al., Allelic effects of mouse Pas1 candidate genes in human lung cancer cell lines. Cancer Lett. 2006 Dec 8;244(2):176-81

Candidate Genes for Pas15:

Gene	Genome Location (GRCm39)	Reference	QTL Note
Bcat1	Chr6:144939561-145021883 (-)	J:90940	The Pas15 lung tumor susceptibility locus was localized to a 486 kb region on mouse Chromosome 6 by fine mapping of the recombinant congenic (RC) strain CXB5/ByJ. CXB5/ByJ is derived from BALB/cJ and C57BL/6J progenitor strains and is highly resistant to lung tumor development. Genotyping of 65 markers in 29 inbred strains for a 1 Mb region around Pas15 revealed a core haplotype that is either C57BL/6-derived or A/J-derived. The Pas1 critical region contains 6 candidate genes: Bcat1, Lrmp, Kras, Casc1, 4933403M22Rik, and 4930469P12Rik. In lung tumors from (A/J x C57BL/6J)F1 mice, Casc1 appears to be expressed solely from the A/J allele and expression of 4933403M22Rik appears to be completely downregulated.
Dnai7	Chr6:145120560-145156731 (-)	J:108212	Previously identified QTL Pas15 (pulmonary adenoma susceptibility 1) maps to a 468 kb interval on mouse Chromosome 6. This region contains six candidate genes: Lrmp, Casc1, 4930469P12Rik (aka Ghiso), and 4933403M22Rik (aka Lmna-rs1). Allele-specific expression constructs of each of the candidate genes were derived from lung mRNA from inbred strains A/J (pulmonary adenoma susceptible) and C57BL/6J (pulmonary adenoma resistant). The expression constructs were transfected into 2 human small-cell lung carcinoma cell lines and assayed for transformation. The ability of Lrmp and 4930469P12Rik constructs to transform human lung carcinoma cells did not differ between A/J and C57BL/6J alleles. However, the A/J allele of Casc1 produced fewer transformed human cells compared to the C57BL/6J allele, and the A/J allele of 4933403M22Rik produced more transformed human cells compared to the C57BL/6J allele. Casc1 was observed preferentially expressed in lung tumors of the A/J-derived allele. 4933403M22Rik expression was not observed in mouse lung tumors of any genotype. Authors state that the experimental results do not necessarily preclude Lrmp and 4930469P12Rik as candidate genes for Pas1.
Dnai7	Chr6:145120560-145156731 (-)	J:90940	The Pas15 lung tumor susceptibility locus was localized to a 486 kb region on mouse Chromosome 6 by fine mapping of the recombinant congenic (RC) strain CXB5/ByJ. CXB5/ByJ is derived from BALB/cJ and C57BL/6J progenitor strains and is highly resistant to lung tumor development. Genotyping of 65 markers in 29 inbred strains for a 1 Mb region around Pas15 revealed a core haplotype that is either C57BL/6-derived or A/J-derived. The Pas1 critical region contains 6 candidate genes: Bcat1, Lrmp, Kras, Casc1, 4933403M22Rik, and 4930469P12Rik. In lung tumors from (A/J x C57BL/6J)F1 mice, Casc1 appears to be expressed solely from the A/J allele and expression of 4933403M22Rik appears to be completely downregulated.
Etfrf1	Chr6:145156860-145162665 (+)	J:90940	The Pas15 lung tumor susceptibility locus was localized to a 486 kb region on mouse Chromosome 6 by fine mapping of the recombinant congenic (RC) strain CXB5/ByJ. CXB5/ByJ is derived from BALB/cJ and C57BL/6J progenitor strains and is highly resistant to lung tumor development. Genotyping of 65 markers in 29 inbred strains for a 1 Mb region around Pas15 revealed a core haplotype that is either C57BL/6-derived or A/J-derived. The Pas1 critical region contains 6 candidate genes: Bcat1, Lrmp, Kras, Casc1, 4933403M22Rik, and 4930469P12Rik. In lung tumors from (A/J x C57BL/6J)F1 mice, Casc1 appears to be expressed solely from the A/J allele and expression of 4933403M22Rik appears to be completely downregulated.
Etfrf1	Chr6:145156860-145162665 (+)	J:108212	Previously identified QTL Pas15 (pulmonary adenoma susceptibility 1) maps to a 468 kb interval on mouse Chromosome 6. This region contains six candidate genes: Lrmp, Casc1, 4930469P12Rik (aka Ghiso), and 4933403M22Rik (aka Lmna-rs1). Allele-specific expression constructs of each of the candidate genes were derived from lung mRNA from inbred strains A/J (pulmonary adenoma susceptible) and C57BL/6J (pulmonary adenoma resistant). The expression constructs were transfected into 2 human small-cell lung carcinoma cell lines and assayed for transformation. The ability of Lrmp and 4930469P12Rik constructs to transform human lung carcinoma cells did not differ between A/J and C57BL/6J alleles. However, the A/J allele of Casc1 produced fewer transformed human cells compared to the C57BL/6J allele, and the A/J allele of 4933403M22Rik produced more transformed human cells compared to the C57BL/6J allele. Casc1 was observed preferentially expressed in lung tumors of the A/J-derived allele. 4933403M22Rik expression was not observed in mouse lung tumors of any genotype. Authors state that the experimental results do not necessarily preclude Lrmp and 4930469P12Rik as candidate genes for Pas1.
Irag2	Chr6:145061379-145120660 (+)	J:108212	Previously identified QTL Pas15 (pulmonary adenoma susceptibility 1) maps to a 468 kb interval on mouse Chromosome 6. This region contains six candidate genes: Lrmp, Casc1, 4930469P12Rik (aka Ghiso), and 4933403M22Rik (aka Lmna-rs1). Allele-specific expression constructs of each of the candidate genes were derived from lung mRNA from inbred strains A/J (pulmonary adenoma susceptible) and C57BL/6J (pulmonary adenoma resistant). The expression constructs were transfected into 2 human small-cell lung carcinoma cell lines and assayed for transformation. The ability of Lrmp and 4930469P12Rik constructs to transform human lung carcinoma cells did not differ between A/J and C57BL/6J alleles. However, the A/J allele of Casc1 produced fewer transformed human cells compared to the C57BL/6J allele, and the A/J allele of 4933403M22Rik produced more transformed human cells compared to the C57BL/6J allele. Casc1 was observed preferentially expressed in lung tumors of the A/J-derived allele. 4933403M22Rik expression was not observed in mouse lung tumors of any genotype. Authors state that the experimental results do not necessarily preclude Lrmp and 4930469P12Rik as candidate genes for Pas1.
Irag2	Chr6:145061379-145120660 (+)	J:90940	The Pas15 lung tumor susceptibility locus was localized to a 486 kb region on mouse Chromosome 6 by fine mapping of the recombinant congenic (RC) strain CXB5/ByJ. CXB5/ByJ is derived from BALB/cJ and C57BL/6J progenitor strains and is highly resistant to lung tumor development. Genotyping of 65 markers in 29 inbred strains for a 1 Mb region around Pas15 revealed a core haplotype that is either C57BL/6-derived or A/J-derived. The Pas1 critical region contains 6 candidate genes: Bcat1, Lrmp, Kras, Casc1, 4933403M22Rik, and 4930469P12Rik. In lung tumors from (A/J x C57BL/6J)F1 mice, Casc1 appears to be expressed solely from the A/J allele and expression of 4933403M22Rik appears to be completely downregulated.
Kras	Chr6:145162425-145195965 (-)	J:90940	The Pas15 lung tumor susceptibility locus was localized to a 486 kb region on mouse Chromosome 6 by fine mapping of the recombinant congenic (RC) strain CXB5/ByJ. CXB5/ByJ is derived from BALB/cJ and C57BL/6J progenitor strains and is highly resistant to lung tumor development. Genotyping of 65 markers in 29 inbred strains for a 1 Mb region around Pas15 revealed a core haplotype that is either C57BL/6-derived or A/J-derived. The Pas1 critical region contains 6 candidate genes: Bcat1, Lrmp, Kras, Casc1, 4933403M22Rik, and 4930469P12Rik. In lung tumors from (A/J x C57BL/6J)F1 mice, Casc1 appears to be expressed solely from the A/J allele and expression of 4933403M22Rik appears to be completely downregulated.
Lmntd1	Chr6:145311619-145560045 (-)	J:90940	The Pas15 lung tumor susceptibility locus was localized to a 486 kb region on mouse Chromosome 6 by fine mapping of the recombinant congenic (RC) strain CXB5/ByJ. CXB5/ByJ is derived from BALB/cJ and C57BL/6J progenitor strains and is highly resistant to lung tumor development. Genotyping of 65 markers in 29 inbred strains for a 1 Mb region around Pas15 revealed a core haplotype that is either C57BL/6-derived or A/J-derived. The Pas1 critical region contains 6 candidate genes: Bcat1, Lrmp, Kras, Casc1, 4933403M22Rik, and 4930469P12Rik. In lung tumors from (A/J x C57BL/6J)F1 mice, Casc1 appears to be expressed solely from the A/J allele and expression of 4933403M22Rik appears to be completely downregulated.
Lmntd1	Chr6:145311619-145560045 (-)	J:108212	Previously identified QTL Pas15 (pulmonary adenoma susceptibility 1) maps to a 468 kb interval on mouse Chromosome 6. This region contains six candidate genes: Lrmp, Casc1, 4930469P12Rik (aka Ghiso), and 4933403M22Rik (aka Lmna-rs1). Allele-specific expression constructs of each of the candidate genes were derived from lung mRNA from inbred strains A/J (pulmonary adenoma susceptible) and C57BL/6J (pulmonary adenoma resistant). The expression constructs were transfected into 2 human small-cell lung carcinoma cell lines and assayed for transformation. The ability of Lrmp and 4930469P12Rik constructs to transform human lung carcinoma cells did not differ between A/J and C57BL/6J alleles. However, the A/J allele of Casc1 produced fewer transformed human cells compared to the C57BL/6J allele, and the A/J allele of 4933403M22Rik produced more transformed human cells compared to the C57BL/6J allele. Casc1 was observed preferentially expressed in lung tumors of the A/J-derived allele. 4933403M22Rik expression was not observed in mouse lung tumors of any genotype. Authors state that the experimental results do not necessarily preclude Lrmp and 4930469P12Rik as candidate genes for Pas1.