Tg(PGK1-FTL*)#Sle
Transgene Detail

Summary

• Symbol: Tg(PGK1-FTL*)#Sle
• Name: transgene insertion, Sonia Levi
• MGI ID: MGI:5700531
• Synonyms: FTL-498InsTC
• Transgene: Tg(PGK1-FTL*)#Sle Location: unknown
• Alliance: Tg(PGK1-FTL*)#Sle page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(PGK1-FTL*)#Sle expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  FTL (2512)

  Ftl1	(MGI:95589)
  Ftl1-ps2	(MGI:5434102)
  Ftl2-ps	(MGI:95590)

• Mutation details: The constitutive and ubiquitous human PGK1 promoter drives the expression of the human cDNA bearing the 498-499InsTC mutation causing the substitution of the last 9 amino acids and an elongation of extra 16 amino acids at the C-terminus of L-ferritin peptide. This modification reduces the ability of the protein to store iron. ELISA and Immunoblotting confirm high expression of the transgene in the liver and brain. Although three lines were obtained, only one was used for further analysis. However, line numbers were not identified and the pound (#) symbol is used because the line number is not specified. (J:227451)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:227451 Maccarinelli F, et al., A novel neuroferritinopathy mouse model (FTL 498InsTC) shows progressive brain iron dysregulation, morphological signs of early neurodegeneration and motor coordination deficits. Neurobiol Dis. 2015 Sep;81:119-33
• All: 2 reference(s)