Tg(Cck-EGFP/RNAi:Gad1)2Mirn
Transgene Detail

Summary

• Symbol: Tg(Cck-EGFP/RNAi:Gad1)2Mirn
• Name: transgene insertion 2, Karoly Mirnics
• MGI ID: MGI:5703888
• Synonyms: CCKGAD1TG, Tg(Cck-eGFP/miRNA:GAD1)2KM
• Transgene: Tg(Cck-EGFP/RNAi:Gad1)2Mirn Location: unknown
• Alliance: Tg(Cck-EGFP/RNAi:Gad1)2Mirn page

Transgene origin

• Strain of Origin: C57BL/6NHsd

Transgene description

• Transgene Type: Transgenic (Conditional ready, Knockdown, Reporter)
• Mutation: Insertion
• Tg(Cck-EGFP/RNAi:Gad1)2Mirn involves 1 genes/genome features (Gad1) View all
• Mutation details: The transgenic construct uses 116 kbp from the bacterial artificial chromosome (BAC) RP23-60I1 containing the cholecystokinin locus (Cck) as well as ~55 kbp of centromeric sequences (including a portion of the Trak1 locus) and ~56 kbp of telomeric sequences; no other confirmed complete genes are present in the flanking sequences. Using homologous recombination/BAC recombineering, the following was inserted in-frame with the start codon of the BAC-encoded Cck: eGFP, a lox2272 site, a human -globin minigene (exon1-intron-exon2) with the intron containing a miRNA:Gad1 sequence, a second lox2272 site and an SV40 polyA signal. An FRT-flanked neomycin resistance cassette was also present, but removed during BAC recombineering in vitro. The miRNA:Gad1 sequence is a fused miR30,miRNA:Gad1 synthetic miRNA sequence directed against the mouse glutamic acid decarboxylase 1 (Gad1 or GAD67) mRNA (mp ID 283874), with unique flanking miR-30 sequences. No other loci on the BAC were altered. (J:227900)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:227900 Schmidt MJ, et al., Modulation of behavioral networks by selective interneuronal inactivation. Mol Psychiatry. 2014 May;19(5):580-7
• All: 1 reference(s)