Tg(Camk2a-Grin2a/Grin2b)1Jzt
Transgene Detail

Summary

• Symbol: Tg(Camk2a-Grin2a/Grin2b)1Jzt
• Name: transgene insertion 1, Joe Z Tsien
• MGI ID: MGI:5780173
• Synonyms: GluN2A^2B(CT), Tg-GluN2A^2B(CTR), Tg-NR2A^2B(CT)
• Transgene: Tg(Camk2a-Grin2a/Grin2b)1Jzt Location: unknown
• Alliance: Tg(Camk2a-Grin2a/Grin2b)1Jzt page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Camk2a-Grin2a/Grin2b)1Jzt expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Grin2a (24409)

  Grin2a

  (MGI:95820)

  rat

  Grin2b (24410)

  Grin2b

  (MGI:95821)

• Mutation details: The transgene is designed to have ~8.5 kbp of the mouse Camk2a promoter sequences placed upstream of a chimeric NMDA glutamate receptor followed by an SV40 polyadenylation signal. Specifically, the N-terminal and transmembrane domain sequences from the rat Grin2a locus are fused with the C-terminal intracellular domain sequences from the rat Grin2b locus. Mice from the founder line with the highest expression in forebrain regions (cortex and hippocampus) were propagated. The investigator reports that Southern blots indicate only one copy of the transgene was integrated into the genome, and there is no evidence of multiple copies. (J:223086)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:223086 Jacobs S, et al., Molecular and genetic determinants of the NMDA receptor for superior learning and memory functions. PLoS One. 2014;9(10):e111865
• All: 1 reference(s)