Tg(Hsf4/EGFP)N7Spbh
Transgene Detail

Summary

• Symbol: Tg(Hsf4/EGFP)N7Spbh
• Name: transgene insertion N7, Suraj P Bhat
• MGI ID: MGI:5810681
• Transgene: Tg(Hsf4/EGFP)N7Spbh Location: unknown
• Alliance: Tg(Hsf4/EGFP)N7Spbh page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(Hsf4/EGFP)N7Spbh expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Hsf4 (MGI:1347058)

• Mutation details: This transgene consists of BAC clone RP24-227N21(170 kb) containing the mouse Hsf4 gene with enhanced green fluorescent coding sequence with a polyadenylation signal sequence inserted at the end of exon 1 of Hsf4 that codes for part of the DNA binding domain and a FRT-Tn5-neo-FRT cassette. Flpe-mediated recombination removed the Tn5-neo cassette. This BAC contains genes between Cbfb and Hspc171. The transgene produces the mRNA for the hybrid gene containing the exon 1 sequences of the Hsf4 gene and the EGFP coding sequences and the mRNA translates into a approximate 32 kDa hybrid protein (N-terminal 41 amino acids of Hsf4 and 239 amino acids EGFP). Four lines (N1, N4, N7 and N8) were generated. Line N7 is entered as a representative line with the most severe phenotype. (J:230205)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:230205 Gangalum RK, et al., Expression of the HSF4 DNA binding domain-EGFP hybrid gene recreates early childhood lamellar cataract in transgenic mice. Invest Ophthalmol Vis Sci. 2014 Nov;55(11):7227-40
• All: 2 reference(s)