Tg(CMV-cat,-ROCK2*)3-1Koba
Transgene Detail

Summary

• Symbol: Tg(CMV-cat,-ROCK2*)3-1Koba
• Name: transgene insertion 3-1, Kazuto Kobayashi
• MGI ID: MGI:5910536
• Synonyms: CAT-Rho-K DN/3-1
• Transgene: Tg(CMV-cat,-ROCK2*)3-1Koba Location: unknown
• Alliance: Tg(CMV-cat,-ROCK2*)3-1Koba page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Conditional ready, Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Tg(CMV-cat,-ROCK2*)3-1Koba expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  cattle	ROCK2 (282041)	mutated to generate a dominant-negative form

• Mutation details: This transgene consists of a 0.9 kb DNA fragment containing the cytomegalovirus early gene promoter fused to the rabbit beta-globin second intron, a loxP flanked chloramphenicol acetyltransferase (cat) cassette connected to a polyadenylation signal, followed by a 1.4 kb DNA fragment encoding a bovine dominant-negative mutant for Rho-associated coiled-coil containing protein kinase 2, which contains two point mutations to suppress Rho-binding activity, and a second polyadenylation signal. Upon cre-mediated excision of the cat gene, the modified allele expresses the mutant ROCK2 in tissues where cre is expressed. Lines 3-1, 3-3, 4-9, 4-11, 5-18, and 5-27 were generated. (J:100207, J:242116)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:100207 Kobayashi K, et al., Survival of developing motor neurons mediated by Rho GTPase signaling pathway through Rho-kinase. J Neurosci. 2004 Apr 7;24(14):3480-8
• All: 9 reference(s)