Tg(KRT5-Terf2)PMBlas
Transgene Detail

Summary

• Symbol: Tg(KRT5-Terf2)PMBlas
• Name: transgene insertion PM, Maria A Blasco
• MGI ID: MGI:6258221
• Synonyms: PM K5-Terf2
• Transgene: Tg(KRT5-Terf2)PMBlas Location: unknown Genetic Position: ChrX, Syntenic
• Alliance: Tg(KRT5-Terf2)PMBlas page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(KRT5-Terf2)PMBlas expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Terf2 (MGI:1195972)

• Tg(KRT5-Terf2)PMBlas expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  cattle	KRT5 (281268)

• Mutation details: The full-length mouse Terf2 cDNA was placed under control of the 5' regulatory region of the bovine keratin K5 promoter, which targets TERF2 to basal cells and stem cells of the epidermis. Three lines were generated and analyzed (PM, PN and PO); a fourth line (PL) had undetectable levels of transgene expression and was not studied further. In the PM line, the transgene is linked to the X chromosome and is selectively silenced in transgenic females. The PM line (males only) showed the highest levels of Terf2 mRNA expression in tail and back skin (~59 times greater than wild-type controls), followed by the PO line (~23 times greater) and the PN line (~12 times greater). Western blot analysis revealed significantly elevated TERF2 protein levels in primary keratinocytes derived from PM and PO transgenic mice. Combined immunofluorescence and telomeric Q-FISH analysis showed that the overexpressed TERF2 protein localized to telomeres. (J:102653)

Disease models

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:102653 Munoz P, et al., XPF nuclease-dependent telomere loss and increased DNA damage in mice overexpressing TRF2 result in premature aging and cancer. Nat Genet. 2005 Oct;37(10):1063-71
• All: 1 reference(s)