Tg(Tyr-Ctnnb1/EGFP)#Lru
Transgene Detail

Summary

• Symbol: Tg(Tyr-Ctnnb1/EGFP)#Lru
• Name: transgene insertion, Lionel Larue
• MGI ID: MGI:6272337
• Synonyms: bcat*, bcat^sta, Tyr::beta-cat*, Tyr::beta-cat-mut-nls-egfp
• Transgene: Tg(Tyr-Ctnnb1/EGFP)#Lru Location: unknown
• Alliance: Tg(Tyr-Ctnnb1/EGFP)#Lru page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Constitutively active, Inserted expressed sequence)
• Mutations: Insertion, Nucleotide substitutions
• Tg(Tyr-Ctnnb1/EGFP)#Lru expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ctnnb1 (MGI:88276)	mouse Tyr enh/promoter drives expression of a cDNA encoding a mutated form of beta-catenin tagged with EGFP and stabilized by four serine/threonine (S/T) to alanine (A) mutations in N-terminal region; a nuclear localization signal restricts protein to the nucleus

• Mutation details: The mouse tyrosinase (Tyr) gene enhancer was fused to the Tyr promoter region to produce a 6.1-kb regulatory element driving the expression of a cDNA encoding a stabilized form of beta-catenin in which Ser33, Ser37, Ser45, and Thr41 were replaced by alanines (A33, A37, A41, A45). A nuclear localization signal (nls) and EGFP sequences were fused in frame to the 3' end of the mutated beta-catenin cDNA. An SV40 small T-antigen splice site and polyadenylation sequence were added to the 3' end of the construct to produce Tyr::beta-cat-mut-nls-egfp (bcat^sta). This expression vector produces a constitutively activated nuclear form of the protein that is comparable in its activity to the mutated form of beta-catenin found in melanomas and other cancers. The transgene was confirmed to be specifically expressed in cells of the melanocyte lineage. Two independent lines (lines 1 and 2) were generated, both presenting a hypopigmented coat color phenotype. The level of transgene expression in melanocytes cultured from newborn skin was ~10% and 5% of that of the endogenous protein in lines 1 and 2, respectively. The lines are pooled, as denoted by the pound (#) sign. (J:127315)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:127315 Delmas V, et al., Beta-catenin induces immortalization of melanocytes by suppressing p16INK4a expression and cooperates with N-Ras in melanoma development. Genes Dev. 2007 Nov 15;21(22):2923-35
• All: 5 reference(s)