Tg(Vav1-Asxl1*Y588X)#Fcy
Transgene Detail

Summary

• Symbol: Tg(Vav1-Asxl1*Y588X)#Fcy
• Name: transgene insertion, Feng-Chun Yang
• MGI ID: MGI:6315194
• Synonyms: Asxl1^Y588XTg
• Transgene: Tg(Vav1-Asxl1*Y588X)#Fcy Location: unknown
• Alliance: Tg(Vav1-Asxl1*Y588X)#Fcy page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Vav1-Asxl1*Y588X)#Fcy expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Asxl1 (MGI:2684063)

• Mutation details: The entire coding region of mouse Asxl1 cDNA with a TAC to TGA change resulting in a stop codon mutation on the Y588 site with a FLAG tag is under the control of the Vav1 promoter. Y558X in the mouse corresponds to human Y591X which is the most frequent nonsense mutation type in patients with malignant myeloid diseases. Western blot analysis confirmed the expression of a truncated protein. Five lines were generated, I through V. The pound symbol (#) is used when no line is specified and/or lines are pooled. J:257690 reports pooled data from lines I and II. (J:257690)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tg(Vav1-Asxl1*Y588X)#Fcy

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:257690 Yang H, et al., Gain of function of ASXL1 truncating protein in the pathogenesis of myeloid malignancies. Blood. 2018 Jan 18;131(3):328-341
• All: 3 reference(s)