Tg(Myh6-MYH7B)#Lnwd
Transgene Detail

Summary

• Symbol: Tg(Myh6-MYH7B)#Lnwd
• Name: transgene insertion, Leslie A Leinwand
• MGI ID: MGI:6712703
• Synonyms: alpha-MYH7b
• Transgene: Tg(Myh6-MYH7B)#Lnwd Location: unknown
• Alliance: Tg(Myh6-MYH7B)#Lnwd page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Epitope tag, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-MYH7B)#Lnwd expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MYH7B (57644)

  Myh7b

  (MGI:3710243)

• Tg(Myh6-MYH7B)#Lnwd expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Myh6 (MGI:97255)

• Mutation details: This transgene construct contains the following elements: mouse myosin heavy chain 6 promoter sequence (also contains last exon of Myh7 and the first three, 5' UTR containing, exons of Myh6) the human myosin heavy chain 7B cDNA (excluding sequence for human-specific 42 aa extension, and with the sequence for the last 10 aa (RDALGPKHKE) replaced with a c-Myc tag (EQKLISEEDL) and human growth hormone gene poly(A) sequences. This allele drives the expression of the human gene in cardiomyocytes, where the Myh7b peptide is not normally expressed. Of the two founder mice, the mouse with the highest expression (~30% of total cardiac myosin) was chosen for further studies. (J:285433)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:285433 Peter AK, et al., Expression of Normally Repressed Myosin Heavy Chain 7b in the Mammalian Heart Induces Dilated Cardiomyopathy. J Am Heart Assoc. 2019 Aug 6;8(15):e013318
• All: 1 reference(s)