Tg(Rr436-GCaMP6*,-mKeima*)3AHmd
Transgene Detail

Summary

• Symbol: Tg(Rr436-GCaMP6*,-mKeima*)3AHmd
• Name: transgene insertion 3A, Hiroshi Hamada
• MGI ID: MGI:7492208
• Synonyms: Tg(Nodal NDE-5HT6/GCaMP6, 5HT6/mKeima)3AHmd
• Transgene: Tg(Rr436-GCaMP6*,-mKeima*)3AHmd Location: unknown
• Alliance: Tg(Rr436-GCaMP6*,-mKeima*)3AHmd page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Tg(Rr436-GCaMP6*,-mKeima*)3AHmd expression driven by 2 genes
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nodal (MGI:97359)
  mouse	Rr436 (MGI:7492210)

• Mutation details: The transgene contains the a crown cell-specific enhancer derived from the mouse Nodal gene driving expression of cilia-targeted GCaMP6 and mKeima reporter genes (5-hydroxytryptamine receptor isoform 6; 5HT6/GCaMP6, 5HT6/mKeima). GCaMP6 contains a RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, and rat calmodulin (CaM) cDNA (aa.2-148). Line 3A was generated. (J:336740)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:336740 Katoh TA, et al., Immotile cilia mechanically sense the direction of fluid flow for left-right determination. Science. 2023 Jan 6;379(6627):66-71
• All: 1 reference(s)