Grid2 Gene Detail

Summary

• Symbol
  Grid2
• Name
  glutamate receptor, ionotropic, delta 2
• Synonyms
  B230104L07Rik, GluRdelta2, tpr

• Feature Type
  protein coding gene
• IDs
  MGI:95813
  NCBI Gene: 14804
• Alliance
  gene page
• Transcription Start Sites
  21 TSS
• Candidate for QTL
  1 QTL

Location &
Maps

• Sequence Map
  Chr6:63232860-64681307 bp, + strand

  From Ensembl annotation of GRCm39
  

• View this region in JBrowse
  

• Genome Browsers
  Ensembl | UCSC | NCBI

• Genetic Map
  Chromosome 6, 29.77 cM
• Mapping Data
  31 experiments

Strain
Comparison

• SNPs within 2kb
  42318 from dbSNP Build 142
• Strain Annotations
  18
• PCR
  3
• RFLP
  3

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_95813	protein coding gene	Chr6:63230118-64681307 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0030827	protein coding gene	Chr6:63092343-64590517 (+)
A/J	MGP_AJ_G0030794	protein coding gene	Chr6:60296038-61752073 (+)
AKR/J	MGP_AKRJ_G0030722	protein coding gene	Chr6:62274558-63724197 (+)
BALB/cJ	MGP_BALBcJ_G0030805	protein coding gene	Chr6:60756468-62163881 (+)
C3H/HeJ	MGP_C3HHeJ_G0030907	protein coding gene	Chr6:120508861-121954777 (-)
C57BL/6NJ	MGP_C57BL6NJ_G0031263	protein coding gene	Chr6:64519928-66041540 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0028455	protein coding gene	Chr6:57718247-59137071 (+)
CAST/EiJ	MGP_CASTEiJ_G0029917	protein coding gene	Chr6:62488521-63965769 (+)
CBA/J	MGP_CBAJ_G0030492	protein coding gene	Chr6:67207811-68764503 (+)
DBA/2J	MGP_DBA2J_G0030644	protein coding gene	Chr6:60045420-61450789 (+)
FVB/NJ	MGP_FVBNJ_G0030597	protein coding gene	Chr6:59589364-60976948 (+)
LP/J	MGP_LPJ_G0030728	protein coding gene	Chr6:63035263-64527451 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0030632	protein coding gene	Chr6:70653319-72520721 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0031289	protein coding gene	Chr6:62208512-63678929 (+)
PWK/PhJ	MGP_PWKPhJ_G0029632	protein coding gene	Chr6:59206016-60623180 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0029460	protein coding gene	Chr6:61694895-63192716 (+)
WSB/EiJ	MGP_WSBEiJ_G0029999	protein coding gene	Chr6:62520164-63961729 (+)

Homology

• Human Ortholog
  GRID2, glutamate ionotropic receptor delta type subunit 2

• Vertebrate Orthologs
  3

Vertebrate Orthology Source

Alliance of Genome Resources

• Human Ortholog
  GRID2, glutamate ionotropic receptor delta type subunit 2
  
• Synonyms
  GluD2, SCAR18
• Links
  HGNC:4576

  NCBI Gene ID: 2895

  neXtProt AC: NX_O43424

  UniProt: O43424

  
  
• Chr Location
  4q22.1-q22.2; chr4:92303966-93810456 (+)  GRCh38

---

• MGI Vertebrate Homology
  Grid2 stringent orthology
  1 human;1 mouse;1 rat;1 zebrafish
  
• HCOP
  vertebrate homology predictions: GRID2
• Gene Tree
  Grid2
  

Human Diseases

• Diseases
  1 with Grid2 mouse models; 1 with human GRID2 associations

Human Disease

Mouse Models

autosomal recessive spinocerebellar ataxia 18

IDs

autosomal recessive spinocerebellar ataxia 18      

DOID:0080042
OMIM:616204

View 2 models

Mouse Models

Human Disease Modeled: autosomal recessive spinocerebellar ataxia 18

Allelic Composition	Genetic Background	Reference	Phenotypes
Grid2tm1(cre)Mwa/Grid2tm1(cre)Mwa	involves: C57BL/6N	J:220519	View
Grid2ho-15J/Grid2ho-15J	involves: C3HeB/Fe	J:220519	View

Click on a disease name to see all genes associated with that disease.

• Mutations/Alleles
  2 with disease annotations

• References
  1 with disease annotations

Mutations,
Alleles, and
Phenotypes

• Phenotype Summary
  108 phenotypes from 30 alleles in 31 genetic backgrounds
  26 phenotypes from multigenic genotypes
  9 images
  235 phenotype references
  

• All Mutations and Alleles
  39
• Chemically induced (ENU)
  3
• Endonuclease-mediated
  3
• Gene trapped
  1
• Spontaneous
  23
• Targeted
  7
• Transgenic
  2
• Incidental Mutations
  Mutagenetix , APF , CvDC
• Find Mice (IMSR)
  85 strains or lines available
• Comparison Matrix
  Gene Expression + Phenotype
• Recombinase Activity
  Comparison Matrix Gene Expression + Recombinase Activity

Homozygotes for multiple spontaneous and targeted null mutations exhibit ataxia and impaired locomotion associated with cerebellar Purkinje cell abnormalities and loss, and on some backgrounds, male infertility due to lack of zona penetration by sperm.

Gene Ontology
(GO)
Classifications

• All GO Annotations
  68
• GO References
  21

Expression

• Assay Results
  804
• Tissues
  86
• cDNA Data
  16
• Literature Summary
  12

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

• Other Mouse Links
  Allen Institute
  GEO
  Expression Atlas
• Other Vertebrate Links
  Xenbase grid2
  ZFIN grid2

Sequences &
Gene Models

• All Sequences
  51
• RefSeq
  6
• UniProt
  3
• CCDS
  CCDS20202.1

• Ensembl
  ENSMUSG00000071424 (Evidence)
• NCBI Gene
  14804

Representative Sequences			Length	Strain/Species
genomic	ENSMUSG00000071424	Ensembl Gene Model | MGI Sequence Detail	1448448	C57BL/6J
transcript	ENSMUST00000095852	Ensembl | MGI Sequence Detail	5860	Not Applicable
polypeptide	ENSMUSP00000093536	Ensembl | MGI Sequence Detail	1007	Not Applicable

Protein
Information

• UniProt
  3 Sequences
• Protein Ontology
  PR:000008238 glutamate receptor ionotropic, delta-2
  (term hierarchy)
• InterPro Domains
  IPR015683 Ionotropic glutamate receptor
  IPR001320 Ionotropic glutamate receptor, C-terminal
  IPR019594 Ionotropic glutamate receptor, L-glutamate and glycine-binding domain
  IPR001508 Ionotropic glutamate receptor, metazoa
  IPR028082 Periplasmic binding protein-like I
  IPR001828 Receptor, ligand binding region
• GlyGen
  Q61625 7 sites, 8 N-linked glycans (4 sites), 1 O-linked glycan (3 sites)

Molecular
Reagents

• All nucleic 37

  Genomic 14

  cDNA 16

  Primer pair 5

  Other 2

  
  

  Microarray probesets 6

Other
Accession IDs

MGD-MRK-10251, MGD-MRK-10810, MGD-MRK-11756, MGD-MRK-2116, MGI:1890672, MGI:2151352, MGI:2442634, MGI:3606121, MGI:96166, MGI:96754

References

• Summaries
  All 325

  Developmental Gene Expression 12

  Diseases 1

  Gene Ontology 21

  Phenotypes 235
• Earliest
  J:289 Phillips RJS, "Lurcher", a new gene in linkage group XI of the house mouse. J Genet. 1960;57(1):35-42
• Latest
  J:351220 Spanaki C, et al., Glutamate-specific gene linked to human brain evolution enhances synaptic plasticity and cognitive processes. iScience. 2024 Feb 16;27(2):108821

TSS for Grid2:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr56779	Chr6:63232861-63232870 (+)	6 bp
Tssr56780	Chr6:63232876-63232887 (+)	22 bp
Tssr56781	Chr6:63232919-63232938 (+)	69 bp
Tssr56782	Chr6:63232955-63233020 (+)	128 bp
Tssr56783	Chr6:63233036-63233053 (+)	185 bp
Tssr56784	Chr6:63233078-63233088 (+)	223 bp
Tssr56785	Chr6:63233091-63233110 (+)	241 bp
Tssr56786	Chr6:63233343-63233365 (+)	494 bp
Tssr56787	Chr6:63233605-63233611 (+)	748 bp
Tssr56788	Chr6:63234791-63234793 (+)	1,932 bp
Tssr56789	Chr6:63234897-63234907 (+)	2,042 bp
Tssr56790	Chr6:63308493-63308496 (+)	75,635 bp
Tssr56791	Chr6:63313718-63313721 (+)	80,860 bp
Tssr56792	Chr6:63493196-63493223 (+)	260,350 bp
Tssr56793	Chr6:63523742-63523744 (+)	290,883 bp
Tssr56794	Chr6:63530106-63530113 (+)	297,250 bp
Tssr56795	Chr6:63556820-63556823 (+)	323,962 bp
Tssr56796	Chr6:63576402-63576407 (+)	343,545 bp
Tssr56797	Chr6:63582419-63582424 (+)	349,562 bp
Tssr56798	Chr6:63723547-63723550 (+)	490,689 bp
Tssr56799	Chr6:63890415-63890424 (+)	657,560 bp

Grid2 is Candidate Gene for:

QTL	Genetic Location*	Genome Location (GRCm39)	Reference	QTL Note
Etax6	Chr6, syntenic	Chr6:71299605-86252701	J:83912	Candidate genes for alcohol traits were identified using gene expression analysis. Total brain RNA from 10 week old male HAFT1 and LAFT1 (and replicate HAFT2 and LAFT2) mice were hybridized to DNA microarrays to detect differentially expressed genes. HAFT1 and LAFT1 are high and low alcohol functional tolerance (AFT) selection lines, respectively, derived from HS/Ibg. HAFT1 mice display greater alcohol functional tolerance and blood ethanol concentration at time of regaining balance on the dowel test compared to LAFT1 mice. Candidate genes mapping to previously identified AFT QTL intervals are described below. Differential expression of candidate genes was also confirmed using RT-PCR analysis. Promoter sequence analysis of identified candidate genes revealed one or more transcription factor binding sites. On mouse Chromosome 1, Prdx6 at 83.6 cM was identified as a potential candidate gene for Etax2 (ethanol induced ataxia 2). This gene is expressed in HAFT1 mice at 87% of the mRNA levels in LAFT1 mice (p<0.001). Etax2 is linked to initial blood ethanol concentration (BEC) after dowel test, acute functional tolerance, and BEC after regaining balance. On mouse Chromosome 2, Grin1 at 12 cM was identified as a potential candidate gene for Etax3 (ethanol induced ataxia 3). This gene isexpressed in HAFT1 mice at 190% of the mRNA levels in LAFT1 mice (p<0.006). Etax3 is linked to acute functional tolerance. On mouse Chromosome 6, Grid2 at 29.65 cM was identified as a potential candidate gene for Etax6 (ethanol induced ataxia 6). Thisgene is expressed in HAFT1 mice at 139% of the mRNA levels in LAFT1 mice (p<0.006). Etax6 is linked to initial BEC after dowel test and BEC after regaining balance. On mouse Chromosome 11, Efnb3 at 40 cM, Zfp179 at 34.5 cM, and Skp1a (formerly Tceb1l) at 31 cM were identified as potential candidate genes for Etax9 (ethanol induced ataxia 9). Efnb3 is expressed in HAFT1 mice at 119% of the mRNA levels in LAFT1 (p<0.002). Zfp197 is expressed at in HAFT1 mice at 159% of the mRNA levels in LAFT1 mice (p<0.002), and Skp1a is expressed at in HAFT1 mice at 137% of the mRNA levels in LAFT1 mice (p<0.002). Etax9 is linked to initial BEC after dowel test and BEC after regaining balance.

*cM position of peak correlated region/marker