Nos2 Gene Detail

Summary

• Symbol
  Nos2
• Name
  nitric oxide synthase 2, inducible
• Synonyms
  iNOS, Nos-2, Nos2a, NOS-II

• Feature Type
  protein coding gene
• IDs
  MGI:97361
  NCBI Gene: 18126
• Alliance
  gene page
• Transcription Start Sites
  95 TSS

Location &
Maps

• Sequence Map
  Chr11:78811613-78851052 bp, + strand

  From NCBI annotation of GRCm39
  

• View this region in JBrowse
  

• Genome Browsers
  Ensembl | UCSC | NCBI

• Genetic Map
  Chromosome 11, 46.74 cM
• Mapping Data
  26 experiments

Strain
Comparison

• SNPs within 2kb
  1290 from dbSNP Build 142
• Strain Annotations
  18
• RFLP
  7

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_97361	protein coding gene	Chr11:78811613-78851080 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0018791	protein coding gene	Chr11:79978936-80021467 (+)
A/J	MGP_AJ_G0018758	protein coding gene	Chr11:76908010-76947472 (+)
AKR/J	MGP_AKRJ_G0018728	protein coding gene	Chr11:79215772-79255173 (+)
BALB/cJ	MGP_BALBcJ_G0018731	protein coding gene	Chr11:77320065-77359027 (+)
C3H/HeJ	MGP_C3HHeJ_G0018545	protein coding gene	Chr11:79263220-79303324 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0019183	protein coding gene	Chr11:82443125-82484262 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0016818	protein coding gene	Chr11:73866986-73905083 (+)
CAST/EiJ	MGP_CASTEiJ_G0018100	protein coding gene	Chr11:79729557-79770158 (+)
CBA/J	MGP_CBAJ_G0018516	protein coding gene	Chr11:86032831-86072186 (+)
DBA/2J	MGP_DBA2J_G0018624	protein coding gene	Chr11:76473641-76513849 (+)
FVB/NJ	MGP_FVBNJ_G0018613	protein coding gene	Chr11:76067304-76107805 (+)
LP/J	MGP_LPJ_G0018694	protein coding gene	Chr11:80497263-80537541 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0018640	protein coding gene	Chr11:86416139-86460683 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0019223	protein coding gene	Chr11:79472471-79514711 (+)
PWK/PhJ	MGP_PWKPhJ_G0017870	protein coding gene	Chr11:77277909-77318456 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0017661	protein coding gene	Chr11:79087804-79129974 (+)
WSB/EiJ	MGP_WSBEiJ_G0018152	protein coding gene	Chr11:79298802-79339346 (+)

Homology

• Human Ortholog
  NOS2, nitric oxide synthase 2

• Vertebrate Orthologs
  4

Vertebrate Orthology Source

Alliance of Genome Resources

• Human Ortholog
  NOS2, nitric oxide synthase 2
  
• Synonyms
  HEP-NOS, INOS, NOS, NOS2A
• Links
  HGNC:7873

  NCBI Gene ID: 4843

  neXtProt AC: NX_P35228

  UniProt: P35228

  
  
• Chr Location
  17q11.2; chr17:27756766-27800529 (-)  GRCh38

---

• MGI Vertebrate Homology
  Nos2 stringent orthology
  1 human;1 mouse;1 rat;2 zebrafish
  
• HCOP
  vertebrate homology predictions: NOS2
• Protein SuperFamily
  NOS
• Gene Tree
  Nos2
  

Human Diseases

• Diseases
  1 with Nos2 mouse models; 8 with human NOS2 associations

Human Disease

Mouse Models

type 1 diabetes mellitus

IDs

type 1 diabetes mellitus      

DOID:9744
ICD10CM:E10
KEGG:04940
MESH:D003922
NCI:C2986
OMIM:222100
UMLS_CUI:C0011854

View 5 models

Mouse Models

Human Disease Modeled: type 1 diabetes mellitus

Allelic Composition	Genetic Background	Reference	Phenotypes
Tg(Ins2-Nos2)40Okam/0 Tg(Kdr-AGER)102Hyam/0	involves: C57BL/6J * CBA/J * CD-1 * DBA/2	J:70495	View
Tg(Ins2-Nos2)40Okam/0	involves: 129P2/OlaHsd * C57BL/6 * CD-1 * DBA/2	J:116040	View
Tg(Ins2-Nos2)31Okam/0	involves: C57BL/6 * CD-1 * DBA/2	J:45520	View
Tg(Ins2-Nos2)40Okam/0	involves: C57BL/6 * CD-1 * DBA/2	J:4552	View
Agertm1.1Hyam/Agertm1.1Hyam Tg(Ins2-Nos2)40Okam/0	involves: 129P2/OlaHsd * C57BL/6 * CD-1 * DBA/2	J:116040	View

asthma

IDs

asthma      

DOID:2841
DOID:12703
DOID:13829
DOID:13830
DOID:2840
DOID:5783
ICD10CM:J45
ICD9CM:493
ICD9CM:493.9
KEGG:05310
MESH:D001249
NCI:C28397
OMIM:600807
UMLS_CUI:C0004096

dementia

IDs

dementia      

DOID:1307
ICD9CM:290.8
UMLS_CUI:C0154319

hypertension

IDs

hypertension      

DOID:10763
EFO:0000537
ICD10CM:I10
ICD9CM:401-405.99
ICD9CM:997.91
MESH:D006973
NCI:C3117
UMLS_CUI:C0020538

Lewy body dementia

IDs

Lewy body dementia      

DOID:12217
ICD10CM:G31.83
ICD9CM_2006:331.82
ICD9CM:331.82
MESH:D020961
NCI:C84826
OMIM:127750
UMLS_CUI:C0752347

malaria

IDs

malaria      

DOID:12365
DOID:12552
EFO:0001068
ICD10CM:B54
ICD9CM:084
ICD9CM:084.6
MESH:D008288
NCI:C34797
ORDO:673
UMLS_CUI:C0024530

pulmonary hypertension

IDs

pulmonary hypertension      

DOID:6432
ICD10CM:I27.20
MESH:D006976
NCI:C3120
UMLS_CUI:C0020542

pulmonary tuberculosis

IDs

pulmonary tuberculosis      

DOID:2957
ICD10CM:A15
ICD9CM:011
ICD9CM:011.9
ICD9CM:011.90
MESH:D014397
NCI:C26899
UMLS_CUI:C0041327

type 2 diabetes mellitus

IDs

type 2 diabetes mellitus      

DOID:9352
ICD10CM:E11
KEGG:04930
MESH:D003924
NCI:C26747
OMIM:125853
OMIM:601283
OMIM:601407
OMIM:603694
OMIM:608036
UMLS_CUI:C0011860

Click on a disease name to see all genes associated with that disease.

• Mutations/Alleles
  4 with disease annotations

• References
  7 with disease annotations

Mutations,
Alleles, and
Phenotypes

• Phenotype Summary
  162 phenotypes from 7 alleles in 17 genetic backgrounds
  103 phenotypes from multigenic genotypes
  1 images
  693 phenotype references
  

• All Mutations and Alleles
  19
• Chemically induced (other)
  1
• Endonuclease-mediated
  6
• Gene trapped
  1
• Targeted
  7
• Transgenic
  4
• Genomic Mutations
  2 involving Nos2
• Incidental Mutations
  Mutagenetix , APF , CvDC
• Find Mice (IMSR)
  67 strains or lines available
• Comparison Matrix
  Gene Expression + Phenotype

Homozygous deletion of this gene alters susceptibility to infection, response to injury, sepsis and sensory stimuli, cardiac function, osteoclast, platelet and mammary gland physiology, tumor growth, testis and uterus morphology, diet-induced atherosclerosis, and blood, urine and glucose metabolism.

Gene Ontology
(GO)
Classifications

• All GO Annotations
  100
• GO References
  27

Expression

• Assay Results
  992
• Tissues
  96
• cDNA Data
  29
• Literature Summary
  49

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

• Other Mouse Links
  Allen Institute
  GEO
  Expression Atlas
• Other Vertebrate Links
  ZFIN nos2a, nos2b

Sequences &
Gene Models

• All Sequences
  48
• RefSeq
  6
• UniProt
  3
• CCDS
  CCDS25115.1

• Ensembl
  ENSMUSG00000020826 (Evidence)
• NCBI Gene
  18126

Representative Sequences			Length	Strain/Species
genomic	18126	NCBI Gene Model | MGI Sequence Detail	39440	C57BL/6J
transcript	NM_010927	RefSeq | MGI Sequence Detail	4164	Not Specified
polypeptide	P29477	UniProt | EBI | MGI Sequence Detail	1144	Not Applicable

Protein
Information

• UniProt
  3 Sequences
• Protein Ontology
  PR:000001901 nitric oxide synthase, inducible
  (term hierarchy)
• PDB
  1DD7, 1DF1, 1DWV, 1DWW, 1DWX, 1JWJ, 1JWK, 1M8D, 1M8E, 1M8H, 1M8I, 1M9T, 1N2N, 1NOC, 1NOD, 1NOS, 1QOM, 1QW4, 1QW5, 1R35, 1VAF, 2BHJ, 2NOD, 2NOS, 2ORO, 2ORP, 2ORQ, 2ORR, 2ORS, 2ORT, 2Y37, 3DWJ, 3E65, 3E67, 3E68, 3E6L, 3E6N, 3E6O, 3E6T, 3E7I, 3E7M, 3E7T, 3EAI, 3EBD, 3EBF, 3GOF, 3NOD, 3NQS, 3NW2, 4JS9, 4UX6
• EC
  1.14.13.39
• InterPro Domains
  IPR017927 FAD-binding domain, ferredoxin reductase-type
  IPR039261 Ferredoxin-NADP reductase (FNR), nucleotide-binding domain
  IPR001094 Flavodoxin-like
  IPR008254 Flavodoxin/nitric oxide synthase
  IPR029039 Flavoprotein-like superfamily
  IPR001709 Flavoprotein pyridine nucleotide cytochrome reductase
  IPR023173 NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamily
  IPR044943 Nitric oxide synthase, domain 1 superfamily
  IPR044940 Nitric oxide synthase, domain 2 superfamily
  IPR044944 Nitric oxide synthase, domain 3 superfamily
  IPR012144 Nitric-oxide synthase, eukaryote
  IPR050607 Nitric Oxide Synthase (NOS)
  IPR004030 Nitric oxide synthase, N-terminal
  IPR036119 Nitric oxide synthase, N-terminal domain superfamily
  IPR001433 Oxidoreductase FAD/NAD(P)-binding
  IPR017938 Riboflavin synthase-like beta-barrel
  IPR003097 Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding
• GlyGen
  P29477 1 site, 1 O-linked glycan (1 site)

Molecular
Reagents

• All nucleic 46

  Genomic 3

  cDNA 32

  Primer pair 8

  Other 3

  
  

  Microarray probesets 3

Other
Accession IDs

MGD-MRK-12910, MGD-MRK-12913

References

• Summaries
  All 1023

  Developmental Gene Expression 49

  Diseases 7

  Gene Ontology 27

  Phenotypes 693
• Earliest
  J:137335 Roderick TH, Chromosomal inversions in studies of mammalian mutagenesis. Genetics. 1979 May;92(1 Pt 1 Suppl):s121-6
• Latest
  J:355438 Ting KKY, et al., Cholesterol accumulation impairs HIF-1alpha-dependent immunometabolic reprogramming of LPS-stimulated macrophages by upregulating the NRF2 pathway. Sci Rep. 2024 May 15;14(1):11162

TSS for Nos2:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr103335	Chr11:78811645-78811660 (+)	40 bp
Tssr103336	Chr11:78813043-78813132 (+)	1,475 bp
Tssr103337	Chr11:78813148-78813165 (+)	1,544 bp
Tssr103338	Chr11:78815036-78815043 (+)	3,427 bp
Tssr103339	Chr11:78816000-78816023 (+)	4,399 bp
Tssr103340	Chr11:78816919-78816931 (+)	5,312 bp
Tssr103341	Chr11:78817375-78817400 (+)	5,775 bp
Tssr103342	Chr11:78818228-78818238 (+)	6,620 bp
Tssr103343	Chr11:78819416-78819425 (+)	7,808 bp
Tssr103344	Chr11:78819426-78819445 (+)	7,823 bp
Tssr103345	Chr11:78820572-78820596 (+)	8,971 bp
Tssr103346	Chr11:78820602-78820614 (+)	8,995 bp
Tssr103347	Chr11:78820646-78820658 (+)	9,039 bp
Tssr103348	Chr11:78820661-78820671 (+)	9,053 bp
Tssr103349	Chr11:78822419-78822427 (+)	10,810 bp
Tssr103350	Chr11:78822436-78822453 (+)	10,832 bp
Tssr103351	Chr11:78822474-78822483 (+)	10,866 bp
Tssr103352	Chr11:78822490-78822505 (+)	10,885 bp
Tssr103353	Chr11:78826176-78826183 (+)	14,567 bp
Tssr103354	Chr11:78826188-78826192 (+)	14,577 bp
Tssr103355	Chr11:78826220-78826245 (+)	14,620 bp
Tssr103356	Chr11:78826248-78826259 (+)	14,641 bp
Tssr103357	Chr11:78826290-78826302 (+)	14,683 bp
Tssr103358	Chr11:78827225-78827249 (+)	15,624 bp
Tssr103359	Chr11:78827255-78827290 (+)	15,660 bp
Tssr103360	Chr11:78827317-78827325 (+)	15,708 bp
Tssr103361	Chr11:78828383-78828398 (+)	16,778 bp
Tssr103362	Chr11:78828634-78828678 (+)	17,043 bp
Tssr103363	Chr11:78828714-78828724 (+)	17,106 bp
Tssr103364	Chr11:78829009-78829017 (+)	17,400 bp
Tssr103365	Chr11:78830827-78830842 (+)	19,222 bp
Tssr103366	Chr11:78830868-78830887 (+)	19,265 bp
Tssr103367	Chr11:78831074-78831088 (+)	19,468 bp
Tssr103368	Chr11:78831089-78831102 (+)	19,483 bp
Tssr103369	Chr11:78831132-78831141 (+)	19,524 bp
Tssr103370	Chr11:78831172-78831181 (+)	19,564 bp
Tssr3591	Chr11:78831398-78831407 (+)	19,790 bp
Tssr103371	Chr11:78831461-78831471 (+)	19,853 bp
Tssr103372	Chr11:78831771-78831803 (+)	20,174 bp
Tssr103373	Chr11:78835494-78835506 (+)	23,887 bp
Tssr103374	Chr11:78835558-78835602 (+)	23,967 bp
Tssr103375	Chr11:78835609-78835625 (+)	24,004 bp
Tssr103376	Chr11:78835634-78835650 (+)	24,029 bp
Tssr103377	Chr11:78836054-78836090 (+)	24,459 bp
Tssr103378	Chr11:78836459-78836482 (+)	24,858 bp
Tssr103379	Chr11:78836495-78836505 (+)	24,887 bp
Tssr103380	Chr11:78836508-78836520 (+)	24,901 bp
Tssr103381	Chr11:78836544-78836578 (+)	24,948 bp
Tssr103382	Chr11:78836695-78836705 (+)	25,087 bp
Tssr103383	Chr11:78836706-78836714 (+)	25,097 bp
Tssr103384	Chr11:78836717-78836724 (+)	25,108 bp
Tssr103385	Chr11:78836729-78836744 (+)	25,124 bp
Tssr103386	Chr11:78838665-78838676 (+)	27,058 bp
Tssr103387	Chr11:78839033-78839059 (+)	27,433 bp
Tssr103388	Chr11:78839120-78839132 (+)	27,513 bp
Tssr103389	Chr11:78839154-78839163 (+)	27,546 bp
Tssr103390	Chr11:78839168-78839183 (+)	27,563 bp
Tssr103391	Chr11:78839884-78839900 (+)	28,279 bp
Tssr103392	Chr11:78839909-78839943 (+)	28,313 bp
Tssr103393	Chr11:78839981-78840001 (+)	28,378 bp
Tssr103394	Chr11:78840002-78840011 (+)	28,394 bp
Tssr103395	Chr11:78840424-78840454 (+)	28,826 bp
Tssr103396	Chr11:78840475-78840489 (+)	28,869 bp
Tssr103397	Chr11:78840779-78840789 (+)	29,171 bp
Tssr103398	Chr11:78840790-78840807 (+)	29,186 bp
Tssr103399	Chr11:78840812-78840834 (+)	29,210 bp
Tssr103400	Chr11:78840840-78840873 (+)	29,244 bp
Tssr103401	Chr11:78840884-78840893 (+)	29,276 bp
Tssr103402	Chr11:78840898-78840922 (+)	29,297 bp
Tssr103403	Chr11:78841929-78841961 (+)	30,332 bp
Tssr103404	Chr11:78841965-78842005 (+)	30,372 bp
Tssr3592	Chr11:78842012-78842018 (+)	30,402 bp
Tssr103405	Chr11:78842026-78842035 (+)	30,418 bp
Tssr103406	Chr11:78842047-78842052 (+)	30,437 bp
Tssr103407	Chr11:78843611-78843624 (+)	32,005 bp
Tssr103408	Chr11:78843631-78843652 (+)	32,029 bp
Tssr103409	Chr11:78843677-78843692 (+)	32,072 bp
Tssr103410	Chr11:78843745-78843758 (+)	32,139 bp
Tssr103411	Chr11:78843792-78843808 (+)	32,187 bp
Tssr103412	Chr11:78843809-78843818 (+)	32,201 bp
Tssr103413	Chr11:78846227-78846237 (+)	34,619 bp
Tssr103414	Chr11:78847350-78847359 (+)	35,742 bp
Tssr103415	Chr11:78848236-78848253 (+)	36,632 bp
Tssr103416	Chr11:78848297-78848310 (+)	36,691 bp
Tssr103417	Chr11:78848331-78848353 (+)	36,729 bp
Tssr103418	Chr11:78848401-78848409 (+)	36,792 bp
Tssr103419	Chr11:78848894-78848902 (+)	37,285 bp
Tssr103420	Chr11:78849527-78849537 (+)	37,919 bp
Tssr103421	Chr11:78849648-78849659 (+)	38,041 bp
Tssr103422	Chr11:78850478-78850489 (+)	38,871 bp
Tssr103423	Chr11:78850508-78850520 (+)	38,901 bp
Tssr103424	Chr11:78850526-78850537 (+)	38,919 bp
Tssr103425	Chr11:78850635-78850702 (+)	39,056 bp
Tssr103426	Chr11:78850713-78850726 (+)	39,107 bp
Tssr103427	Chr11:78850747-78850757 (+)	39,139 bp