Pnlip Gene Detail

Summary

• Symbol
  Pnlip
• Name
  pancreatic lipase
• Synonyms
  1810007A24Rik, MGC:74173, pancreatic triglyceride lipase, PTL

• Feature Type
  protein coding gene
• IDs
  MGI:97722
  NCBI Gene: 69060
• Alliance
  gene page
• Transcription Start Sites
  32 TSS
• Candidate for QTL
  6 QTL

Location &
Maps

• Sequence Map
  Chr19:58658797-58670231 bp, + strand

  From NCBI annotation of GRCm39
  

• View this region in JBrowse
  

• Genome Browsers
  Ensembl | UCSC | NCBI

• Genetic Map
  Chromosome 19, 54.46 cM, cytoband D3
• Mapping Data
  9 experiments

Strain
Comparison

• SNPs within 2kb
  402 from dbSNP Build 142
• Strain Annotations
  18
• RFLP
  2

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_97722	protein coding gene	Chr19:58640930-58670231 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0025278	protein coding gene	Chr19:59158151-59171051 (+)
A/J	MGP_AJ_G0025254	protein coding gene	Chr19:56257804-56269296 (+)
AKR/J	MGP_AKRJ_G0025223	protein coding gene	Chr19:58015801-58027156 (+)
BALB/cJ	MGP_BALBcJ_G0025249	protein coding gene	Chr19:56320347-56333024 (+)
C3H/HeJ	MGP_C3HHeJ_G0025009	protein coding gene	Chr19:58263595-58276128 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0025692	protein coding gene	Chr19:60580972-60596597 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0023104	protein coding gene	Chr19:55135165-55146415 (+)
CAST/EiJ	MGP_CASTEiJ_G0024474	protein coding gene	Chr19:58336493-58350201 (+)
CBA/J	MGP_CBAJ_G0024989	protein coding gene	Chr19:63530367-63548491 (+)
DBA/2J	MGP_DBA2J_G0025121	protein coding gene	Chr19:56001395-56013111 (+)
FVB/NJ	MGP_FVBNJ_G0025082	protein coding gene	Chr19:55739863-55751160 (+)
LP/J	MGP_LPJ_G0025207	protein coding gene	Chr19:59014076-59026602 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0025115	protein coding gene	Chr19:62786268-62799652 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0025750	protein coding gene	Chr19:58244474-58257798 (+)
PWK/PhJ	MGP_PWKPhJ_G0024222	protein coding gene	Chr19:56004717-56016375 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0024025	protein coding gene	Chr19:56909544-56920709 (+)
WSB/EiJ	MGP_WSBEiJ_G0024544	protein coding gene	Chr19:58363976-58379325 (+)

Homology

• Human Ortholog
  PNLIP, pancreatic lipase

• Vertebrate Orthologs
  2

Vertebrate Orthology Source

Alliance of Genome Resources

• Human Ortholog
  PNLIP, pancreatic lipase
  
• Synonyms
  PL, PNLIPD, PTL
• Links
  HGNC:9155

  NCBI Gene ID: 5406

  neXtProt AC: NX_P16233

  UniProt: P16233

  
  
• Chr Location
  10q25.3; chr10:116545931-116567855 (+)  GRCh38

---

• MGI Vertebrate Homology
  Pnlip stringent orthology
  1 human;1 mouse;1 rat
  
• HCOP
  vertebrate homology predictions: PNLIP
• Protein SuperFamily
  Lipoprotein_lipase_LIPH
• Gene Tree
  Pnlip
  

Mutations,
Alleles, and
Phenotypes

• Phenotype Summary
  11 phenotypes from 1 allele in 2 genetic backgrounds
  9 phenotypes from multigenic genotypes
  8 phenotype references
  

• All Mutations and Alleles
  7
• Endonuclease-mediated
  3
• Targeted
  4
• Incidental Mutations
  Mutagenetix , CvDC
• Find Mice (IMSR)
  28 strains or lines available
• Comparison Matrix
  Gene Expression + Phenotype

Homozygous null mice show impaired dietary cholesterol, triglyceride and retinyl ester absorption, resistance to diet-induced obesity, and insulin resistance.

Gene Ontology
(GO)
Classifications

• All GO Annotations
  22
• GO References
  9

Expression

• Assay Results
  806
• Tissues
  97
• cDNA Data
  333
• Literature Summary
  8

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

• Other Mouse Links
  Allen Institute
  GEO
  Expression Atlas
• Other Vertebrate Links
  Xenbase pnlip

Sequences &
Gene Models

• All Sequences
  36
• RefSeq
  2
• UniProt
  2
• CCDS
  CCDS29930.1

• Ensembl
  ENSMUSG00000046008 (Evidence)
• NCBI Gene
  69060

Representative Sequences			Length	Strain/Species
genomic	69060	NCBI Gene Model | MGI Sequence Detail	11435	C57BL/6J
transcript	NM_026925	RefSeq | MGI Sequence Detail	1498	Not Specified
polypeptide	Q6P8U6	UniProt | EBI | MGI Sequence Detail	465	Not Applicable

Protein
Information

• UniProt
  2 Sequences
• Protein Ontology
  PR:000012925 pancreatic triacylglycerol lipase
  (term hierarchy)
• EC
  3.1.1.3
• InterPro Domains
  IPR029058 Alpha/Beta hydrolase fold
  IPR013818 Lipase
  IPR016272 Lipase, LIPH-type
  IPR033906 Lipase, N-terminal
  IPR002331 Pancreatic lipase
  IPR001024 PLAT/LH2 domain
  IPR036392 PLAT/LH2 domain superfamily
  IPR000734 Triacylglycerol lipase family

Molecular
Reagents

• All nucleic 336

  cDNA 335

  Other 1

  
  

  Microarray probesets 5

Other
Accession IDs

MGD-MRK-13544, MGI:1916310

References

• Summaries
  All 50

  Developmental Gene Expression 8

  Gene Ontology 9

  Phenotypes 8
• Earliest
  J:43376 Grusby MJ, et al., Cloning of an interleukin-4 inducible gene from cytotoxic T lymphocytes and its identification as a lipase. Cell. 1990 Feb 9;60(3):451-9
• Latest
  J:337474 Cobo I, et al., NFIC regulates ribosomal biology and ER stress in pancreatic acinar cells and restrains PDAC initiation. Nat Commun. 2023 Jun 23;14(1):3761

TSS for Pnlip:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr157258	Chr19:58658605-58658639 (+)	-175 bp
Tssr157259	Chr19:58658782-58658787 (+)	-12 bp
Tssr157260	Chr19:58658790-58658800 (+)	-2 bp
Tssr157261	Chr19:58658857-58658862 (+)	63 bp
Tssr157262	Chr19:58658865-58658893 (+)	82 bp
Tssr157263	Chr19:58660133-58660153 (+)	1,346 bp
Tssr157264	Chr19:58660161-58660211 (+)	1,389 bp
Tssr157265	Chr19:58660217-58660263 (+)	1,443 bp
Tssr157266	Chr19:58661504-58661517 (+)	2,714 bp
Tssr157267	Chr19:58661518-58661536 (+)	2,730 bp
Tssr5571	Chr19:58661553-58661559 (+)	2,759 bp
Tssr157268	Chr19:58661576-58661590 (+)	2,786 bp
Tssr157269	Chr19:58662173-58662183 (+)	3,381 bp
Tssr157270	Chr19:58662236-58662257 (+)	3,450 bp
Tssr157271	Chr19:58663488-58663497 (+)	4,696 bp
Tssr157272	Chr19:58663499-58663513 (+)	4,709 bp
Tssr157273	Chr19:58664630-58664660 (+)	5,848 bp
Tssr157274	Chr19:58664662-58664663 (+)	5,866 bp
Tssr157275	Chr19:58664682-58664695 (+)	5,892 bp
Tssr157276	Chr19:58664705-58664721 (+)	5,916 bp
Tssr157277	Chr19:58664946-58664954 (+)	6,153 bp
Tssr157278	Chr19:58665568-58665582 (+)	6,778 bp
Tssr157279	Chr19:58665585-58665600 (+)	6,796 bp
Tssr157280	Chr19:58665601-58665649 (+)	6,828 bp
Tssr157281	Chr19:58667956-58667969 (+)	9,166 bp
Tssr157282	Chr19:58667974-58667989 (+)	9,185 bp
Tssr157283	Chr19:58668176-58668181 (+)	9,382 bp
Tssr157284	Chr19:58668466-58668489 (+)	9,681 bp
Tssr157285	Chr19:58669166-58669194 (+)	10,383 bp
Tssr157286	Chr19:58669237-58669241 (+)	10,442 bp
Tssr157287	Chr19:58669254-58669269 (+)	10,465 bp
Tssr157288	Chr19:58670178-58670189 (+)	11,387 bp

Pnlip is Candidate Gene for:

QTL	Genetic Location*	Genome Location (GRCm39)	Reference	QTL Note
Afw8	Chr19, syntenic		J:99477	Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes. Potential candidate genes for Iba4 (20 cM) on mouse Chromosome 19 as identified by ExQuest are Aldh1a1 (12 cM), Aldh1a7, Vldlr (20 cM), and Plce1. Vldlr exhibits 15.6-fold increased expression in the liver of Tally Ho compared to C57BL/6 animals. For QTL Afw8 (26 cM), potential candidate genes Pi4k2a, Cpn1, and Elovl3 (47 cM) were identified. For QTLs Nobq2 (47 cM) and Bglq13 (51 cM), potential candidate genes Ins1 (49 cM), Gpam (52 cM), and Acsl5 were identified. For QTLs Tanidd1 (50 cM) and T2dm2 (53 cM), potential candidate genes Gfra1, Pnlip (29 cM), Pnliprp1 (29 cM), and Pnliprp2 were identified. Pnliprp2 exhibits 21.8-fold decreased expression in the pancreas of Tally Ho animals in response to a 4% fat diet.
Bglq13	Chr19, syntenic		J:99477	Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes. Potential candidate genes for Iba4 (20 cM) on mouse Chromosome 19 as identified by ExQuest are Aldh1a1 (12 cM), Aldh1a7, Vldlr (20 cM), and Plce1. Vldlr exhibits 15.6-fold increased expression in the liver of Tally Ho compared to C57BL/6 animals. For QTL Afw8 (26 cM), potential candidate genes Pi4k2a, Cpn1, and Elovl3 (47 cM) were identified. For QTLs Nobq2 (47 cM) and Bglq13 (51 cM), potential candidate genes Ins1 (49 cM), Gpam (52 cM), and Acsl5 were identified. For QTLs Tanidd1 (50 cM) and T2dm2 (53 cM), potential candidate genes Gfra1, Pnlip (29 cM), Pnliprp1 (29 cM), and Pnliprp2 were identified. Pnliprp2 exhibits 21.8-fold decreased expression in the pancreas of Tally Ho animals in response to a 4% fat diet.
Iba4	Chr19, 20.99 cM	Chr19:26305715-26305821	J:99477	Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes. Potential candidate genes for Iba4 (20 cM) on mouse Chromosome 19 as identified by ExQuest are Aldh1a1 (12 cM), Aldh1a7, Vldlr (20 cM), and Plce1. Vldlr exhibits 15.6-fold increased expression in the liver of Tally Ho compared to C57BL/6 animals. For QTL Afw8 (26 cM), potential candidate genes Pi4k2a, Cpn1, and Elovl3 (47 cM) were identified. For QTLs Nobq2 (47 cM) and Bglq13 (51 cM), potential candidate genes Ins1 (49 cM), Gpam (52 cM), and Acsl5 were identified. For QTLs Tanidd1 (50 cM) and T2dm2 (53 cM), potential candidate genes Gfra1, Pnlip (29 cM), Pnliprp1 (29 cM), and Pnliprp2 were identified. Pnliprp2 exhibits 21.8-fold decreased expression in the pancreas of Tally Ho animals in response to a 4% fat diet.
Nobq2	Chr19, 40.53 cM	Chr19:47897880-47897988	J:99477	Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes. Potential candidate genes for Iba4 (20 cM) on mouse Chromosome 19 as identified by ExQuest are Aldh1a1 (12 cM), Aldh1a7, Vldlr (20 cM), and Plce1. Vldlr exhibits 15.6-fold increased expression in the liver of Tally Ho compared to C57BL/6 animals. For QTL Afw8 (26 cM), potential candidate genes Pi4k2a, Cpn1, and Elovl3 (47 cM) were identified. For QTLs Nobq2 (47 cM) and Bglq13 (51 cM), potential candidate genes Ins1 (49 cM), Gpam (52 cM), and Acsl5 were identified. For QTLs Tanidd1 (50 cM) and T2dm2 (53 cM), potential candidate genes Gfra1, Pnlip (29 cM), Pnliprp1 (29 cM), and Pnliprp2 were identified. Pnliprp2 exhibits 21.8-fold decreased expression in the pancreas of Tally Ho animals in response to a 4% fat diet.
T2dm2	Chr19, 50.72 cM	Chr19:55376050-55376167	J:99477	Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes. Potential candidate genes for Iba4 (20 cM) on mouse Chromosome 19 as identified by ExQuest are Aldh1a1 (12 cM), Aldh1a7, Vldlr (20 cM), and Plce1. Vldlr exhibits 15.6-fold increased expression in the liver of Tally Ho compared to C57BL/6 animals. For QTL Afw8 (26 cM), potential candidate genes Pi4k2a, Cpn1, and Elovl3 (47 cM) were identified. For QTLs Nobq2 (47 cM) and Bglq13 (51 cM), potential candidate genes Ins1 (49 cM), Gpam (52 cM), and Acsl5 were identified. For QTLs Tanidd1 (50 cM) and T2dm2 (53 cM), potential candidate genes Gfra1, Pnlip (29 cM), Pnliprp1 (29 cM), and Pnliprp2 were identified. Pnliprp2 exhibits 21.8-fold decreased expression in the pancreas of Tally Ho animals in response to a 4% fat diet.
Tanidd1	Chr19, 48.46 cM	Chr19:53752597-53752718	J:99477	Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes. Potential candidate genes for Iba4 (20 cM) on mouse Chromosome 19 as identified by ExQuest are Aldh1a1 (12 cM), Aldh1a7, Vldlr (20 cM), and Plce1. Vldlr exhibits 15.6-fold increased expression in the liver of Tally Ho compared to C57BL/6 animals. For QTL Afw8 (26 cM), potential candidate genes Pi4k2a, Cpn1, and Elovl3 (47 cM) were identified. For QTLs Nobq2 (47 cM) and Bglq13 (51 cM), potential candidate genes Ins1 (49 cM), Gpam (52 cM), and Acsl5 were identified. For QTLs Tanidd1 (50 cM) and T2dm2 (53 cM), potential candidate genes Gfra1, Pnlip (29 cM), Pnliprp1 (29 cM), and Pnliprp2 were identified. Pnliprp2 exhibits 21.8-fold decreased expression in the pancreas of Tally Ho animals in response to a 4% fat diet.

*cM position of peak correlated region/marker