pDO-beta-2 Probe Detail MGI Mouse MGI:10296

pDO-beta-2 Probe Detail

Name

pDO-beta-2
Sequence Type

cDNA
ID

MGI:10296
Region Covered

encodes J[b]2.6, C[b]2 and 3' untranslated region
Child Clone

C-beta-2-specific probe
Insert Site

HindIII/EcoRI
Insert Size

~0.8 kb
Note

Hybridizes to C[b]1 and C[b]2 sequences.

Species

mouse, laboratory
Tissue

T-lymphocyte
Cell Line

hybridoma cell line DO.11.10

Tcrb	T cell receptor beta chain
Tcrb-C	T cell receptor beta, constant region
Tcrb-J	T cell receptor beta, joining region

J:7920 Kotzin BL, et al., Science. 1985 Jul 12;229(4709):167-71

Endonuclease	Gene	Allele	Fragments	Strains
BamHI	Tcrb	a	~8.0,~6.6kb	SJL
		c	~9.4,~6.6kb	BALB/c, NZB
		n	~8.0kb	NZW
EcoRI	Tcrb	a	9.4,~4kb	SJL
		c	9.4,~3kb	BALB/c, NZB
		n	9.4kb	NZW
HindIII	Tcrb	c	~9.4,~3.5kb	BALB/c, NZB, SJL
HindIII	Tcrb	n	~9.0kb	NZW
HpaII	Tcrb	a	9.4,6.6kb	SJL
		c	9.4,6.6kb	BALB/c, NZB
		n	~8.0kb	NZW
PvuII	Tcrb	a	~8.0,~11.0kb	SJL
		c	6.0,6.2kb	BALB/c, NZB
		n	5.0kb	NZW

J:8689 Kotzin BL, et al., J Exp Med. 1987 May 1;165(5):1237-51

Notes: The two NZB restriction fragments represent the C[b]1 and C[b]2 gene segments. The 5.0 kb NZW fragment represents C[b]2 only (NZW is deleted for C[b]1).

Endonuclease	Gene	Allele	Fragments	Strains
PvuII	Tcrb	c	6.0,6.2kb	NZB
PvuII	Tcrb	n	5.0kb	NZW

J:7843 Born W, et al., Rearrangement of T-cell receptor beta-chain genes during T-cell development. Proc Natl Acad Sci U S A. 1985 May;82(9):2925-9

J:7920 Kotzin BL, et al., A large deletion within the T-cell receptor beta-chain gene complex in New Zealand white mice. Science. 1985 Jul 12;229(4709):167-71

J:8689 Kotzin BL, et al., The contribution of NZW genes to lupus-like disease in (NZB x NZW)F1 mice. J Exp Med. 1987 May 1;165(5):1237-51

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 10/29/2024 MGI 6.24