KB5 Probe Detail MGI Mouse MGI:10673

KB5 Probe Detail

Name

KB5
Sequence Type

genomic
ID

MGI:10673
Region Covered

encodes 5' flanking sequence, Igh-V, Igh-D, Igh-J (J4) gene segments and 3' flank sequence

Species

mouse, laboratory
Strain

MRL-Fas
Cell Line

hybridoma cell line

Igh-D	immunoglobulin heavy chain diversity region
Igh-J	immunoglobulin heavy chain, joining region
Igh-V	immunoglobulin heavy chain variable region

J:7743 Trepicchio W Jr, et al., J Immunol. 1985 Apr;134(4):2734-9

Notes: A 1.5 kb fragment was isolated from KB5 and used in Southern analysis. As a result of this probe encoding Igh-J (J4) and its 3' flanking sequence, a 6.6 kb fragment containing the unrearranged Igh-J gene segments is observed in all strains tested.

Endonuclease	Gene	Allele	Fragments	Strains
EcoRI	Igh-V	a	multiple	BALB/c, RIII
		b	multiple	C57BL/6
		c	multiple	DBA/2
		d	multiple	SWR
		e	multiple	AKR, NZB
		f	multiple	C3H, LG, MRL, MRL-Fas^lpr

J:7743 Trepicchio W Jr, et al., The Igh-V locus of MRL mice: restriction fragment length polymorphism in eleven strains of mice as determined with VH and D gene probes. J Immunol. 1985 Apr;134(4):2734-9

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