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pMPa21 Probe Detail
Nucleotide
Probe/Clone
  • Name
    pMPa21
  • Sequence Type
    cDNA
  • ID
    MGI:11950
  • Region Covered
    pMPa21 is a full length cDNA
  • Child Clone
    Amy cDNA
  • Vector Type
    Plasmid
  • Insert Site
    PstI
  • Insert Size
    1577 bp
  • Note
    pMPa21 hybridizes to both Amy1 and Amy2.
Source
  • Species
    mouse, laboratory
  • Strain
    A/J
  • Tissue
    pancreas
Genes
Amy2a5 amylase 2a5
Amy2a2 amylase 2a2
Amy2a4 amylase 2a4
Amy2a3 amylase 2a3
Sequences
J00360 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)
V00718 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)
Polymorphisms
J:7469 Thomsen KK, et al., Biochem Genet. 1984 Apr;22(3-4):257-73
Notes: Alleles share many common restriction fragments however band intensities vary.
Endonuclease Gene Allele Fragments Strains
EcoRI Amy2a5 a 5.4,1.7kb A/J, BALB/c, C3H/As
b not given RIIIS/J
w15 not given C3.Amyw15
w16 12.0,1.7kb C3.Amyw16
J:9139 Paul PR, et al., Biochem Genet. 1987 Aug;25(7-8):569-79
Notes: Strains CE/J, YBR/Ki and MOLD/RkA display unique patterns in BglII and HindIII Southern blots (fragment sizes not given).
Endonuclease Gene Allele Fragments Strains
BglII Amy2a5 b 4.2kb 129P3/J, BUB/BnJ, C57BL/6J, C57BL/10J, C57BR/cdJ, C57L/J, C58/J, DW/J, FS/EiJ, LP/J, NZB/BlNJ, O20/A, SB/LeJ, SM/J
d 4.4kb A/J, ABP/LeJ, AKR/FuRdA, AKR/J, AU/SsJ, BALB/cByJ, BDP/J, C3H/HeJ, C3HeB/FeJ, C57BLKS/J, CBA/CaJ, CBA/J, DBA/1J, DBA/2DeJ, DBA/2J, GRS/A, I/LnJ, LG/J, MA/MyJ, P/J, PL/J, RF/J, SEC/1ReJ, SJL/J, ST/bJ, STS/A, STX/Le, SWR/J, WB/ReJ
HindIII Amy2a5 b 6.3kb 129P3/J, BUB/BnJ, C57BL/6J, C57BL/10J, C57BR/cdJ, C57L/J, C58/J, DW/J, FS/EiJ, LP/J, NZB/BlNJ, O20/A, SB/LeJ, SM/J
d 8.1kb A/J, ABP/LeJ, AKR/FuRdA, AKR/J, AU/SsJ, BALB/cByJ, BDP/J, C3H/HeJ, C3HeB/FeJ, C57BLKS/J, CBA/CaJ, CBA/J, DBA/1J, DBA/2DeJ, DBA/2J, GRS/A, I/LnJ, LG/J, MA/MyJ, P/J, PL/J, RF/J, SEC/1ReJ, SJL/J, ST/bJ, STS/A, STX/Le, SWR/J, WB/ReJ
PstI Amy2a5 b 6.1kb C57BL/6J, C57L/J, C58/J, NZB/BlNJ, SM/J
d 6.4kb A/J, AKR/J, BALB/cByJ, C3H/HeJ, DBA/2J, SWR/J
References
J:6365 Hagenbuchle O, et al., Tissue-specific expression of mouse-alpha-amylase genes: nucleotide sequence of isoenzyme mRNAs from pancreas and salivary gland. Cell. 1980 Aug;21(1):179-87
J:6405 Schibler U, et al., Tissue-specific expression of mouse alpha-amylase genes. J Mol Biol. 1980 Sep 5;142(1):93-116
J:7469 Thomsen KK, et al., Genetic variation in restriction patterns among mouse amylase gene complexes. Biochem Genet. 1984 Apr;22(3-4):257-73
J:8356 Meisler MH, et al., Interstrain variation in amylase gene copy number and mRNA abundance in three mouse tissues. Genetics. 1986 Jul;113(3):713-22
J:9139 Paul PR, et al., Analysis of the mouse Amy locus in recombinant inbred mouse strains. Biochem Genet. 1987 Aug;25(7-8):569-79
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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