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pHPT2 Probe Detail
Nucleotide
Probe/Clone
  • Name
    pHPT2
  • Sequence Type
    cDNA
  • ID
    MGI:12051
  • Region Covered
    encodes most of the cDNA-missing a small 5' portion
  • Vector Type
    Plasmid
  • Insert Site
    PstI
  • Insert Size
    1100 bp
  • Note
    Although cloned into a PstI site of pBR322, PstI digestion fails to excise the insert. HpaII will release the insert with minor vector sequence contamination (Proc Natl Acad Sci USA 79:1950).
Source
  • Species
    mouse, laboratory
  • Tissue
    neuroblast
  • Cell Line
    neuroblastoma cell line NBR4
Genes
Hprt1 hypoxanthine phosphoribosyltransferase 1
Hprt-ps1 hypoxanthine guanine phosphoribosyl transferase pseudogene 1
Sequences
J00423 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)
References
J:20062 Brennand J, et al., Cloned cDNA sequences of the hypoxanthine/guanine phosphoribosyltransferase gene from a mouse neuroblastoma cell line found to have amplified genomic sequences. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1950-4
J:20083 Konecki DS, et al., Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants. Nucleic Acids Res. 1982 Nov 11;10(21):6763-75
J:9273 Isamat M, et al., Characterization, evolutionary relationships, and chromosome location of processed mouse HPRT pseudogene. Somat Cell Mol Genet. 1988 Jul;14(4):359-69
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/17/2024
MGI 6.24 		The Jackson Laboratory