About   Help   FAQ
pSAM-1 Probe Detail
Nucleotide
Probe/Clone
  • Name
    pSAM-1
  • Sequence Type
    genomic
  • ID
    MGI:12317
  • Region Covered
    encodes the functional Aprt gene, exons 1-5
  • Parent Clone
    pMaprt-11
  • Vector Type
    Plasmid
  • Insert Site
    EcoRI/SphI
  • Insert Size
    3.1 kb
  • Synonyms
    pSAM3.1
Source
  • Species
    mouse, laboratory
  • Strain
    BALB/c
  • Sex
    Male
  • Tissue
    sperm
Genes
Aprt adenine phosphoribosyl transferase
Aprt-ps adenine phosphoribosyl transferase, pseudogene
Polymorphisms
J:8422 Farber RA, et al., Cytogenet Cell Genet. 1986;42(4):198-201
Notes: The polymorphic fragments 6.1 kb (C57BL/6J) and 3.2 kb (AKR/J) result from a RFLV in the Aprt-ps on chromosome 8. The 7.9 kb band contains the functional Aprt gene and the remaining bands are thought to represent other pseudogenes.
Endonuclease Gene Allele Fragments Strains
BglII Aprt-ps a 7.9,5.9,4.9,3.2kb AKR/J
b 7.9,6.1,5.9,4.9kb C57BL/6J
J:3742 Abonia JP, et al., Mamm Genome. 1993;4(1):25-32
Notes: A 3.1kb EcoRI/SalI fragment was used for hybridization.
Endonuclease Gene Allele Fragments Strains
BglI Aprt a 2.7,2.5kb BALB/cByJ, C3H/HeJ, C57BL/6J
m 4.2,2.7kb PWK
KpnI Aprt a 5.3kb BALB/cByJ, C3H/HeJ, C57BL/6J
t 6.8,5.4kb STS/A
References
J:7132 Sikela JM, et al., Cloning and expression of a mouse adenine phosphoribosyltransferase gene. Gene. 1983 May-Jun;22(2-3):219-28
J:7839 Dush MK, et al., Nucleotide sequence and organization of the mouse adenine phosphoribosyltransferase gene: presence of a coding region common to animal and bacterial phosphoribosyltransferases that has a variable intron/exon arrangement. Proc Natl Acad Sci U S A. 1985 May;82(9):2731-5
J:8422 Farber RA, et al., Assignment of a processed mouse Aprt pseudogene to the same chromosome as the functional gene. Cytogenet Cell Genet. 1986;42(4):198-201
J:8539 Dush MK, et al., An unusual adenine phosphoribosyltransferase pseudogene is syntenic with its functional gene and is flanked by highly polymorphic DNAs. Mol Cell Biol. 1986 Dec;6(12):4161-7
J:3742 Abonia JP, et al., Linkage of Agt and Actsk-1 to distal mouse chromosome 8 loci: a new conserved linkage. Mamm Genome. 1993;4(1):25-32
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory