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pLy-5-68 Probe Detail
Nucleotide
Probe/Clone
  • Name
    pLy-5-68
  • Sequence Type
    cDNA
  • ID
    MGI:12353
  • Region Covered
    most of coding region and 3'-flanking sequence
  • Vector Type
    Plasmid
  • Insert Site
    PstI/BamHI
  • Insert Size
    4.5kb
  • Synonyms
    Ly5-68
Source
  • Species
    mouse, laboratory
  • Tissue
    T-lymphocyte
  • Cell Line
    C1.Ly1-T1
Genes
Ptprc protein tyrosine phosphatase receptor type C
Sequences
M11934 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)
Polymorphisms
J:8080 Shen FW, et al., Proc Natl Acad Sci U S A. 1985 Nov;82(21):7360-3
Notes: PstI, PvuII and BamHI only distiguished alleles a and b. Allele c gave the same pattern as allele a using PstI or PvuII. Allele c gave the same pattern as allele b using BamHI. This entry has corrected allele designations from those presented in the reference.
Endonuclease Gene Allele Fragments Strains
BglI Ptprc a several SJL/J, STS/A
b several A, BALB/c, C57BL/6
c several ST/bJ
EcoRI Ptprc a several SJL/J, STS/A
b several A, BALB/c, C57BL/6
c several ST/bJ
HindIII Ptprc a several SJL/J, STS/J
b several A, BALB/c, C57BL/6
c several ST/bJ
J:8805 Seldin MF, et al., Immunogenetics. 1987;26(1-2):74-8
Notes: A 2400 BamHI fragment was isolated from pLy-5-68 and used in Southern analysis. Additional common fragments are seen. Other wild mice were typed with unique alleles. Band sizes are approximations.
Endonuclease Gene Allele Fragments Strains
BamHI Ptprc a 12,5kb DD, RIII/AnN, RIIIS/J, SJL/J, STS/A, STS/J
b 23,9.4kb 129P3/J, A/HeJ, A/J, ABP/LeJ, AKR/A, AL/N, AU/SsJ, BALB/cA, BALB/cJ, BDP/J, BSVS, BUB/BnJ, C3H/HeJ, C57BL/6J, C57BL/10J, C57BR/cdJ, C57L/J, C58/J, CBA/J, CE/J, DBA/2J, HRS/J, I/LnJ, MA/MyJ, MRL/MpJ-Faslpr/J, NFS/N, NZB/BlNJ, NZW/N, O20, P/N, PDB/Pt, PL/J, RF/J, SEA/GnJ, SEC/1ReJ, SJL/JLwPt, SM/J, SWR/J, YBR/EiJ
c 12,5.2,5kb CZECHII, NH, ST/bJ
d 12,5kb CZECHII
e 12,5kb M. m. castaneus
f 12,5kb M. m. molossinus
s 12,5kb M. spretus
EcoRI Ptprc a 9.4,8,3.2kb DD, RIII/AnN, RIIIS/J, SJL/J, STS/A, STS/J
b 9.4,8,5,3.2kb 129P3/J, A/HeJ, A/J, ABP/LeJ, AKR/A, AL/N, AU/SsJ, BALB/cA, BALB/cJ, BDP/J, BSVS, BUB/BnJ, C3H/HeJ, C57BL/6J, C57BL/10J, C57BR/cdJ, C57L/J, C58/J, CBA/J, CE/J, DBA/2J, HRS/J, I/LnJ, MA/MyJ, MRL/MpJ-Faslpr/J, NFS/N, NZB/BlNJ, NZW/N, O20, P/N, PDB/Pt, PL/J, RF/J, SEA/GnJ, SEC/1ReJ, SJL/JLwPt, SM/J, SWR/J, YBR/EiJ
c 9.4,5,3.2kb CZECHII, NH, ST/bJ
d several CZECHII
e several M. m. castaneus
f several M. m. molossinus
s 8,5kb M. spretus
J:9230 Seldin MF, et al., Genomics. 1988 Jan;2(1):48-56
Notes: Allele sizes are estimated from gel. A 2400 bp BamHI fragment derived from pLy-5-68 was used for Southern analysis.
Endonuclease Gene Allele Fragments Strains
EcoRI Ptprc a 9.4,8.0,6.7,3.0,2.6kb DD, RIIIS/J, SJL/J, STS/A, STS/J
b 9.4,8,6.7,5.0,3.0,2.6kb 129P3/J, A/He, A/J, ABP/LeJ, AKR/J, AL/N, BALB/c, BDP/J, BSVS, BUB/BnJ, C3H/Fg, C3H/HeJ, C3H/HeN, C57BL/6J, C57BL/10J, C57BR/cdJ, C57L/J, C58/J, CBA/J, CBA/N, CE/J, DBA/1J, DBA/2J, HRS/J, I/LnJ, MA/MyJ, MRL/MpJ, NFS, NZB/N, O20, RF/J, SEA/GnJ, SEC/1ReJ, SJL/JLwPt, SM/J, SWR/J, YBR/EiJ
c 9.4,7.0,6.7,5.0,3.0,2.6kb NH, ST/bJ
J:9342 Seldin MF, et al., Curr Top Microbiol Immunol. 1988;137:227-32
Endonuclease Gene Allele Fragments Strains
Not Specified Ptprc c not given C3H/HeJ-Faslgld/J
s not given M. spretus
J:10189 Barnum SR, et al., Biochemistry. 1989 Oct 17;28(21):8312-7
Notes: A 2.4 kb BamHI fragment was isolated from pLy-5-68 and was used in Southern analysis. Only diagnostic fragments are listed.
Endonuclease Gene Allele Fragments Strains
BamHI Ptprc c absent C3H-Faslgld
s ~10,4.3kb M. spretus
J:11327 Cox RD, et al., Genomics. 1991 Jun;10(2):375-84
Endonuclease Gene Allele Fragments Strains
BamHI Ptprc b 23.0,10.0,7.4,6.0,4.2,3.0kb C57BL/6J
s 10.4,7.8,5.3,4.3,3.0kb M. spretus
J:23912 Sugiyama S, et al., Eur J Biochem. 1995 Feb 15;228(1):120-8
Notes: A 2.1kb HindIII fragment of Ly5-68 was used in Southern hybridization.
Endonuclease Gene Allele Fragments Strains
BamHI Ptprc n not given NFS/N
s not given M. spretus
References
J:8080 Shen FW, et al., Cloning of Ly-5 cDNA. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7360-3
J:8412 Saga Y, et al., Sequences of Ly-5 cDNA: isoform-related diversity of Ly-5 mRNA [published erratum appears in Proc Natl Acad Sci U S A 1987 Apr;84(7):1991]. Proc Natl Acad Sci U S A. 1986 Sep;83(18):6940-4
J:8805 Seldin MF, et al., Allelic variants of Ly-5 in inbred and natural populations of mice. Immunogenetics. 1987;26(1-2):74-8
J:9230 Seldin MF, et al., Establishment of a molecular genetic map of distal mouse chromosome 1: further definition of a conserved linkage group syntenic with human chromosome 1q. Genomics. 1988 Jan;2(1):48-56
J:9342 Seldin MF, et al., A molecular genetic approach to gld autoimmune disease. Curr Top Microbiol Immunol. 1988;137:227-32
J:10189 Barnum SR, et al., Molecular analysis of the murine C4b-binding protein gene. Chromosome assignment and partial gene organization. Biochemistry. 1989 Oct 17;28(21):8312-7
J:11327 Cox RD, et al., Interspersed repetitive element polymerase chain reaction product mapping using a mouse interspecific backcross. Genomics. 1991 Jun;10(2):375-84
J:23912 Sugiyama S, et al., Cloning and expression of the mouse laminin gamma 2 (B2t) chain, a subunit of epithelial cell laminin. Eur J Biochem. 1995 Feb 15;228(1):120-8
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