pC21/3c Probe Detail MGI Mouse MGI:12356

pC21/3c Probe Detail

Name

pC21/3c
Sequence Type

cDNA
ID

MGI:12356
Region Covered

encodes the coding sequence and untranslated regions
Vector Type

Plasmid
Insert Size

2 kb

Species

human
Age

embryonic
Tissue

adrenal gland

Cyp21a1

cytochrome P450, family 21, subfamily a, polypeptide 1

J:8970 Gillet D, et al., Immunogenetics. 1988;27(2):133-6

Notes: Using pC21/3c to probe EcoRI digested DNA results in 4 distinguishable patterns (alleles designated c and d and those designated b and e give the same patterns). Using TaqI, 5 distinguishable patterns are seen (alleles disignated a and b give the same pattern). Using PvuII, 2 patterns are seen (alleles a, b, c, e, f give the same pattern).

Endonuclease	Gene	Allele	Fragments	Strains
EcoRI	Cyp21a1	a	3.5,3.8,30kb	B10.G-H2^q, C3.B10-H2^b
		b	3.5,3.8,180kb	B10.D2-H2^d, B10.PL-H2^u
		c	3.5,4.0,180kb	B10.M-H2^f/Sn, B10.P-H2^p, B10.WB-H2^ja/Sn, C3H
		d	3.5,4,180kb	B10.RIII-H2^r/Sn
		e	3.5,3.8,180kb	B10.S-H2^s/J
		f	3.5,4,30kb	B10.SM-H2^v
PvuII	Cyp21a1	a	2.2kb	B10.G-H2^q, C3.B10-H2^b
		b	2.2kb	B10.D2-H2^d, B10.PL-H2^u
		c	2.2kb	B10.M-H2^f/Sn, B10.P-H2^p, B10.WB-H2^ja/Sn, C3H
		d	2.2,2.4kb	B10.RIII-H2^r/Sn
		e	2.2kb	B10.S-H2^s/J
		f	2.2kb	B10.SM-H2^v
TaqI	Cyp21a1	a	3.1,3.4kb	B10.G-H2^q, C3.B10-H2^b
		b	3.1,3.4kb	B10.D2-H2^d, B10.PL-H2^u
		c	3.4,3.9kb	B10.M-H2^f/Sn, B10.P-H2^p, B10.WB-H2^ja/Sn, C3H
		d	3.2,3.4kb	B10.RIII-H2^r/Sn
		e	3.4,3.6kb	B10.S-H2^s/J
		f	3.1,3.4,3.9kb	B10.SM-H2^v

J:11154 Walsh AC, et al., Immunogenetics. 1991;33(4):290-3

Endonuclease	Gene	Allele	Fragments	Strains
BamHI	Cyp21a1	b	3.8,3.6kb	B6.R4, B10.RIII-H2^r/(20R), C57BL/6, C57BL/6-H2-T18^a
BamHI	Cyp21a1	r	3.8kb	B6.R1, B6.R2, RIIIS/J

J:20110 White PC, et al., Structure of human steroid 21-hydroxylase genes. Proc Natl Acad Sci U S A. 1986 Jul;83(14):5111-5

J:8970 Gillet D, et al., Extensive genomic polymorphism in mouse 21-hydroxylase region. Immunogenetics. 1988;27(2):133-6

J:11154 Walsh AC, et al., Characterization of several new MHC congenic strains. Immunogenetics. 1991;33(4):290-3

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