C8.5v-k probe Probe Detail MGI Mouse MGI:12568

C8.5v-k probe Probe Detail

Name

C8.5v-k probe
Sequence Type

cDNA
ID

MGI:12568
Region Covered

full length cDNA encoding the lgk-20 gene segment, C8.5 and lgk-J and lgk-C gene segments
Vector Type

Plasmid
Note

Subcloned from a [l]gt10 vector.

Species

mouse, laboratory
Strain

BALB/c
Cell Line

hybridoma cell line C8.5

Igkc	immunoglobulin kappa constant
Igk-J	immunoglobulin kappa chain, joining region
Igk-V20	immunoglobulin kappa chain variable 20 (V20 family)

M55318 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)

J:10673 Shefner R, et al., J Immunol. 1990 Sep 1;145(5):1609-14

Notes: a 182bp Hinf I fragment was derived from C58.Vk probe and used in Southern analysis

Endonuclease	Gene	Allele	Fragments	Strains
BamHI	Igk-V20	a	~7.0,~4.0,~3.3,~3.0kb	A/J, MRL-Fas^lpr
		b	~7.0,~6.5,~4.0,~3.6,~3.0kb	NZB
		c	~7.0,~6.5,~4.0,~3.3,~3.0kb	BALB/c, NZW
EcoRI	Igk-V20	a	~20.0,~9.0,~7.0,~5.0,~4.5kb	(SI/Col x M. spretus)F1 x SI/Col or SI/Col x M. m. musculus)F1 x SI/Col, AKR/J, BALB/cJ, C3H/HeJ, C57BL/6J, DBA/2J, STS/A, SWR/J
		o	~15,~7,~5,~4.5,~4,~3.5kb	O20/A
		s	~20,~15,~9,~7,~5kb	SJL/J
HindIII	Igk-V20	a	~10,~6.5,~2.2,~1kb	A/J, MRL-Fas^lpr
		b	~10,~8.7,~6.5,~6,~2.2,~1kb	NZB
		c	~10,~8.7,~6.5,~2.2,~1kb	BALB/c, NZW

J:10673 Shefner R, et al., Identification of a new V kappa gene family that is highly expressed in hybridomas from an autoimmune mouse strain [see comments]. J Immunol. 1990 Sep 1;145(5):1609-14

J:30242 Zocher I, et al., Clustered and interspersed gene families in the mouse immunoglobulin kappa locus. Eur J Immunol. 1995 Dec;25(12):3326-31

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