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pCP12 Probe Detail
Nucleotide
Probe/Clone
  • Name
    pCP12
  • Sequence Type
    cDNA
  • ID
    MGI:13311
  • Region Covered
    encodes 5' untranslated sequence, Igh-V11 (VCP12), Igh-D (DSP2-type) gene segments and the 5'-portion of an Igh-J gene segment (J1)
  • Vector Type
    Plasmid
  • Insert Site
    PstI
  • Insert Size
    500 bp
Source
  • Species
    mouse, laboratory
  • Strain
    BALB/c
  • Cell Line
    hybridoma cell line CP8 B3-D3
Genes
Igh-D immunoglobulin heavy chain diversity region
Igh-J immunoglobulin heavy chain, joining region
Igh-V11 immunoglobulin heavy chain (V11 family)
Polymorphisms
J:9467 Reininger L, et al., Eur J Immunol. 1988 Oct;18(10):1521-6
Notes: A 261bp DraI/PstI fragment was isolated from pCP12 and used in Southern analysis.
Endonuclease Gene Allele Fragments Strains
BamHI Igh-V11 a 23,~8.5kb BALB/c, DBA/2
b ~18,~4kb C57BL/6
d ~12kb C3H, NZB
EcoRI Igh-V11 a 8.1,8.7kb BALB/c
b ~9.1,~14kb C57BL/6
c ~4.0,8.1,8.7kb DBA/2
d 8.7kb C3H, NZB
HindIII Igh-V11 a 9.4,~5.8kb BALB/c, C57BL/6, DBA/2
b ~7.2kb C3H, NZB
References
J:9467 Reininger L, et al., A member of a new VH gene family encodes antibromelinized mouse red blood cell autoantibodies. Eur J Immunol. 1988 Oct;18(10):1521-6
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory