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PC3386 Probe Detail
Nucleotide
Probe/Clone
  • Name
    PC3386
  • Sequence Type
    genomic
  • ID
    MGI:13757
  • Region Covered
    encodes V10, J pseudogene, J3 and C segments
  • Child Clone
    Igk-V10 genomic1
  • Vector Type
    Phage
  • Insert Site
    BamHI
  • Insert Size
    8.5kb
  • Note
    PC3386 encodes a nonproductive allele of Igk-C, Igk-J, and Igk-V10.
Source
  • Species
    mouse, laboratory
  • Strain
    NZB
  • Tissue
    plasmacytoma
Genes
Igkc immunoglobulin kappa constant
Igk-J immunoglobulin kappa chain, joining region
Igk-V10 immunoglobulin kappa chain variable 10 (V10)
Polymorphisms
J:9125 Seldin MF, et al., Immunogenetics. 1988;27(5):396-8
Endonuclease Gene Allele Fragments Strains
BamHI Igk-V10 d 5.0,8.0,11.0kb C3H/HeJ-Faslgld/J
s 5.0,6.7,8.0,11.0kb M. spretus
J:9339 D'Hoostelaere L, et al., Curr Top Microbiol Immunol. 1988;137:116-29
Endonuclease Gene Allele Fragments Strains
BamHI Igk-V10 a multiple AKR, C58, PL
b multiple ABP, BALB/c, C57BL/6, CE, NZB, SJL
c multiple O20
J:9361 Rueff-Juy D, et al., Immunogenetics. 1988;28(4):233-9
Notes: Only the diagnostic band is listed.
Endonuclease Gene Allele Fragments Strains
EcoRI Igk-V10 m absent MAI
s 4.0,3.0kb SJL
J:17764 Yang JN, et al., J Virol. 1991 Jul;65(7):3911-4
Notes: A 900bp EcoRI/HindIII subclone of PC3386 was used for Southern blots.
Endonuclease Gene Allele Fragments Strains
EcoRI Igk-V10 b ~4.4,~3.0 C57BL/6
c ~7.0,~5.0,~3.2,~2.8kb C58
References
J:7956 Kelley DE, et al., Nonproductive kappa immunoglobulin genes: recombinational abnormalities and other lesions affecting transcription, RNA processing, turnover, and translation. Mol Cell Biol. 1985 Jul;5(7):1660-75
J:9125 Seldin MF, et al., Mapping of the Ly-4 (L3T4) T-cell differentiation antigen on mouse chromosome 6 by the use of RFLPs in an interspecific cross. Immunogenetics. 1988;27(5):396-8
J:9339 D'Hoostelaere L, et al., The organization of the immunoglobulin kappa locus in mice. Curr Top Microbiol Immunol. 1988;137:116-29
J:9361 Rueff-Juy D, et al., Mapping of Igk-V genes using backcrossed laboratory and wild mice. Immunogenetics. 1988;28(4):233-9
J:17764 Yang JN, et al., The endogenous Mtv-8 provirus resides within the V kappa locus. J Virol. 1991 Jul;65(7):3911-4
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