Clone 9 Probe Detail MGI Mouse MGI:15354

Clone 9 Probe Detail

Name

Clone 9
Sequence Type

cDNA
ID

MGI:15354
Region Covered

most of coding region and 3' untranslated sequence
Child Clone

Prn-p cDNA
Vector Type

Phage
Insert Site

EcoRI
Insert Size

1.7 kb
Note

Clone 9 consists of a 0.8 kb fragment (subclone 9-9) and a 0.9 kb fragment (subclone 9-13) joined at an EcoRI site. Subclone 9-13 is also referred to as cDNA fragment number 13. The insert from Clone 9 was subcloned into pEMBL8.

Species

mouse, laboratory
Strain

Swiss
Tissue

brain
Tissue Description

scrapie infected brain

Prnp

prion protein

M30384 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)

J:8918 Westaway D, et al., Cell. 1987 Nov 20;51(4):651-62

Notes: A 0.9 kb fragment (cDNA fragment 13) derived from Clone 9 was used for Southern analysis. Additional restiction site polymorphisms between strains NZW and I/Ln (XbaI, PstI, and EcoRI) lie 3' to exon 2.

Endonuclease	Gene	Allele	Fragments	Strains
BstEII	Prnp	a	5.7,3.5kb	NZW
BstEII	Prnp	b	15.5kb	I/Ln, IM, P/J

J:7876 Chesebro B, et al., Identification of scrapie prion protein-specific mRNA in scrapie-infected and uninfected brain. Nature. 1985 May 23-29;315(6017):331-3

J:8406 Locht C, et al., Molecular cloning and complete sequence of prion protein cDNA from mouse brain infected with the scrapie agent. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6372-6

J:8918 Westaway D, et al., Distinct prion proteins in short and long scrapie incubation period mice. Cell. 1987 Nov 20;51(4):651-62

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 10/29/2024 MGI 6.24