probe 9 Probe Detail MGI Mouse MGI:16827

probe 9 Probe Detail

Name

probe 9
Sequence Type

genomic
ID

MGI:16827
Region Covered

encodes sequence ~60 kb 5' of H-2Ea
Parent Clone

clone 55.2
Insert Site

Sau3A
Insert Size

800 bp

Species

mouse, laboratory
Strain

BALB/c
Sex

Male
Tissue

sperm

H2-Ea

histocompatibility 2, class II antigen E alpha

J:6885 Steinmetz M, et al., Nature. 1982 Nov 4;300(5887):35-42

Notes: Probe 9 detects polymorphic fragments ~60 kb 5' of H-2Ea.

Endonuclease	Gene	Allele	Fragments	Strains
HindIII	H2-Ea	b	18kb	BALB/c
HindIII	H2-Ea	c	14kb	A, C3H, C57BL/6

J:15532 Steinmetz M, et al., EMBO J. 1984 Dec 1;3(12):2995-3003

Notes: This probe detects polymorphic fragments located ~60 kb 5' of H-2Ea.

Endonuclease	Gene	Allele	Fragments	Strains
EcoRI	H2-Ea	a	12.3,7.3kb	A.SW-H2^s/Sn, AKR, B10.DRB62, B10.STA10, BALB/c, C57BL/10
EcoRI	H2-Ea	b	17.2,12.3kb	B10.CAS2, B10.KPA44, B10.STA62
HindIII	H2-Ea	a	14.8kb	A.SW-H2^s/Sn, AKR, B10.CAS2, B10.DRB62, B10.KPA44, B10.STA10, B10.STA62, C57BL/10
HindIII	H2-Ea	b	18.0kb	BALB/c

J:6885 Steinmetz M, et al., A molecular map of the immune response region from the major histocompatibility complex of the mouse. Nature. 1982 Nov 4;300(5887):35-42

J:15532 Steinmetz M, et al., Tracts of high or low sequence divergence in the mouse major histocompatibility complex. EMBO J. 1984 Dec 1;3(12):2995-3003

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