This help document answers the following questions about the Probe and Primer Detail report:
See also:
How do I interpret the probe details?
Each report includes a subset of the following fields, depending on the content of the record. See Primer Detail Results if the sequence type is primer.
| FIELD | DESCRIPTION |
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| Nucleotide Probe/Clone |
| Name | Name of the probe or segment. |
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| Sequence Type | Sequence types are: cDNA, genomic, DNA (construct), mitochondrial, oligo, primer, and Not Specified. |
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| ID | Unique MGI ID assigned to the probe/clone record in MGI. The format is MGI:nnnn (nnnn is any number greater than or equal to 1). |
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| Region Covered | Description of the molecular segment inserted into the vector |
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| Parent Clone | Name of probe/clone that the currently displayed probe/clone is derived from. Each name is linked to the appropriate parent record. |
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| Child Clone | Probes/clones derived from the currently displayed probe/clone. Each name is linked to the appropriate child record. |
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| Vector Type | BAC, cosmid, P1, PAC, phage, phagemid, plasmid, YAC |
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| Insert Site | Restriction site used to insert the segment into the vector |
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| Insert Size | Size of the segment inserted into the vector |
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| Other IDs | A list of any secondary MGI accession IDs associated with the probe/clone as well as unique identifiers assigned to the probe/clone by a resource external to the database. Within MGI, ATCC, and IMAGE and RIKEN clone IDs are examples of external accession IDs. These are linked to the appropriate resource when possible. See also: What clones IDs may be associated with a probe? |
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| Note | Probe-specific information |
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| Synonyms | Alternative names for the clone used in publications |
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|
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| Source |
| Library | Library to which the probe/clone belongs |
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| Species | Organism from which the probe/clone was derived |
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| Strain | Strain from which the probe/clone was derived |
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| Age | Age of the specimen from which the probe/clone was derived |
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| Sex | Sex of the specimen from which the probe/clone was derived |
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| Tissue | Tissue of the specimen from which the probe/clone was derived |
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| Tissue Description | If this is a cell line, the description may detail where the cell line was derived from. If this is a tissue, it may detail the cell type or tissue treatment, if any. |
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| Cell line | Cell line from which the probe/clone was derived |
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|
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| Genes |
- Symbol and name for each gene (linked to MGI gene records) to which this probe/clone hybridizes
- If the probe is putatively associated with a gene in the MGI database, PUTATIVE appears in parentheses after the gene name.
|
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| Expression | Links to expression results derived using the probe |
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| Sequences | For molecular segments associated with sequence accession numbers, the Accession IDs and links to GenBank, EMBL, DDBJ, and the MGI Sequence Detail page |
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| Polymorphisms |
For references with polymorphism data, the short-form citation for this probe/clone, linked via an MGI Reference ID (the J:####) to the Reference Detail page. If polymorphism information is available, it appears in a table with the following column heads:
| Endonuclease | Restriction enzyme used to cut the DNA. |
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| Gene | The gene or marker the primers amplify |
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| Allele | Designation for the locus allele in which the polymorphism was
found. |
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| Fragments | Description of the restriction fragments and PCR products |
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| Strains | Strain names for the strains in which the given polymorphism was detected. |
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|
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| References |
Citations for this probe/clone, each linked via an MGI Reference ID (the J:####) to the Reference Detail page |
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How do I interpret the primer details?
If you selected primer as the Sequence Type on a Molecular Probes and Clones Summary page, the first section of the table, Primers, may contain the information the following information as well as information contained in the
detail report above.
| FIELD | DESCRIPTION |
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| Name | Name of the primer set. |
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| Primer 1 Sequence | Sequence for the first primer. |
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| Primer 2 Sequence | Sequence for the second primer. |
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| ID | Unique MGI ID assigned to the probe/clone record in MGI. The format is MGI:nnnn (nnnn is any number greater than or equal to 1). |
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| Region Covered | Description of the molecular segment amplified by the primers |
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| Product Size | Size of the amplified product. |
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| Other IDs | A list of any secondary MGI accession IDs associated with the probe/clone as well as unique identifiers assigned to the probe/clone by a resource external to the database. Within MGI, the UniSTS ID is an example of an external accession ID. This are linked to the appropriate resource when possible. See also: What clones IDs may be associated with a probe? |
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| Note | Primer-specific information |
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What clones IDs may be associated with a probe?
Potential clone IDs are as follows:
| CLONE/PROBE RESOURCE | LINK |
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| ATCC | |
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| BROAD | |
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| IMAGE | Links to the I.M.A.G.E. Consortium Home Page with information on ordering clones |
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| MGC | Links to the Mammalian Gene Collection Home Page with information on ordering clones |
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| NCBI UniSTS | Links to the NCBI STS probe report |
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| NIA | |
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| NIA 7.4K | |
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| NIA 15K | |
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| RIKEN | Links to the RIKEN clone sequence information |
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| RIKEN (FANTOM) | Links to the RIKEN clone details along with a link for ordering clones |
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| RPCI-23 | Links to the NCBI Clone Summary with links to distributors |
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| RPCI-24 | Links to the NCBI Clone Summary with links to distributors |
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| WashU | |
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Interpreting Probe and Primer Details